Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A
I tiakina i:
| Kaituhi matua: | |
|---|---|
| Rā whakaputa: | 2019 |
| Ētahi atu kaituhi: | , , , , , , |
| Hōputu: | Article |
| Reo: | eng |
| Puna: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/36247 https://doi.org/10.1590/0037-8682-0001-2018 http://orcid.org/0000-0002-4181-142X |
Whakarāpopototanga: | INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis. |
| _version_ | 1871442283929272320 |
|---|---|
| author | Fraiha, Rafael Ovando |
| author2 | Pereira, Ana Paula Ramos Brito, Eliana da Costa Alvarenga Borges, Clayton Luiz Parente, Ana Flávia Alves Perdomo, Renata Trentin Macedo, Maria Ligia Rodrigues Weber, Simone Schneider |
| author2_role | author author author author author author author |
| author_browse | Borges, Clayton Luiz Brito, Eliana da Costa Alvarenga Fraiha, Rafael Ovando Macedo, Maria Ligia Rodrigues Parente, Ana Flávia Alves Perdomo, Renata Trentin Pereira, Ana Paula Ramos Weber, Simone Schneider |
| author_facet | Fraiha, Rafael Ovando Pereira, Ana Paula Ramos Brito, Eliana da Costa Alvarenga Borges, Clayton Luiz Parente, Ana Flávia Alves Perdomo, Renata Trentin Macedo, Maria Ligia Rodrigues Weber, Simone Schneider |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/36247/1/ARTIGO_StressConditionsHost.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Fraiha, Rafael Ovando Pereira, Ana Paula Ramos Brito, Eliana da Costa Alvarenga Borges, Clayton Luiz Parente, Ana Flávia Alves Perdomo, Renata Trentin Macedo, Maria Ligia Rodrigues Weber, Simone Schneider |
| dc.date.accessioned.fl_str_mv | 2020-01-24T10:29:59Z |
| dc.date.available.fl_str_mv | 2020-01-24T10:29:59Z |
| dc.date.issued.fl_str_mv | 2019 |
| dc.identifier.citation.fl_str_mv | FRAIHA, Rafael Ovando et al. Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 52, e20180001, 2019. DOI: https://doi.org/10.1590/0037-8682-0001-2018. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100608. Acesso em: 23 jan. 2020. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.1590/0037-8682-0001-2018 |
| dc.identifier.orcid.none.fl_str_mv | http://orcid.org/0000-0002-4181-142X |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/36247 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Medicina Tropical - SBMT |
| dc.rights.driver.fl_str_mv | OPEN ACCESS - https://creativecommons.org/licenses/by/4.0/ (CC BY) info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Persistência Inanição Virulência (Microbiologia) |
| dc.title.pt_BR.fl_str_mv | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_3bee2514b64e2dfd0e55c9c68e1d0840 |
| identifier_str_mv | FRAIHA, Rafael Ovando et al. Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 52, e20180001, 2019. DOI: https://doi.org/10.1590/0037-8682-0001-2018. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100608. Acesso em: 23 jan. 2020. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/36247 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Sociedade Brasileira de Medicina Tropical - SBMT |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| rights_invalid_str_mv | OPEN ACCESS - https://creativecommons.org/licenses/by/4.0/ (CC BY) |
| spelling | Fraiha, Rafael OvandoPereira, Ana Paula RamosBrito, Eliana da Costa AlvarengaBorges, Clayton LuizParente, Ana Flávia AlvesPerdomo, Renata TrentinMacedo, Maria Ligia RodriguesWeber, Simone Schneider2020-01-24T10:29:59Z2020-01-24T10:29:59Z2019FRAIHA, Rafael Ovando et al. Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 52, e20180001, 2019. DOI: https://doi.org/10.1590/0037-8682-0001-2018. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100608. Acesso em: 23 jan. 2020.https://repositorio.unb.br/handle/10482/36247https://doi.org/10.1590/0037-8682-0001-2018http://orcid.org/0000-0002-4181-142XSociedade Brasileira de Medicina Tropical - SBMTOPEN ACCESS - https://creativecommons.org/licenses/by/4.0/ (CC BY)info:eu-repo/semantics/openAccessStress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62Ainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePersistênciaInaniçãoVirulência (Microbiologia)INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_StressConditionsHost.pdfapplication/pdf1153620http://repositorio2.unb.br/jspui/bitstream/10482/36247/1/ARTIGO_StressConditionsHost.pdfa5505210b09e44096bc0a979cbca0eddMD51open access10482/362472023-05-24 20:34:31.742open accessoai:repositorio.unb.br:10482/36247Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:31Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A Fraiha, Rafael Ovando Persistência Inanição Virulência (Microbiologia) |
| status_str | publishedVersion |
| title | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| title_full | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| title_fullStr | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| title_full_unstemmed | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| title_short | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| title_sort | Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
| topic | Persistência Inanição Virulência (Microbiologia) |
| url | https://repositorio.unb.br/handle/10482/36247 https://doi.org/10.1590/0037-8682-0001-2018 http://orcid.org/0000-0002-4181-142X |