A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease
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| Data wydania: | 2016 |
| Kolejni autorzy: | , , , , , , , , , , , , |
| Format: | Article |
| Język: | eng |
| Źródło: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/24200 https://dx.doi.org/10.1371/journal.pntd.0004581 |
Streszczenie: | Background Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immuneresponses. Methods/Principal Findings Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva.We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. |
| _version_ | 1871442221618692096 |
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| author | Santiago, Paula Beatriz |
| author2 | Assumpção, Teresa Cristina França de Araújo, Carla Nunes de Bastos, Izabela Marques Dourado Neves, David Silva, Ionizete Garcia da Charneau, Sébastien Queiroz, Rayner Myr Lauterjung Raiol, Tainá Oliveira, João Victor de Araújo Sousa, Marcelo Valle de Calvo, Eric Ribeiro, José M. C. Santana, Jaime Martins de |
| author2_role | author author author author author author author author author author author author author |
| author_browse | Araújo, Carla Nunes de Assumpção, Teresa Cristina França de Bastos, Izabela Marques Dourado Calvo, Eric Charneau, Sébastien Neves, David Oliveira, João Victor de Araújo Queiroz, Rayner Myr Lauterjung Raiol, Tainá Ribeiro, José M. C. Santana, Jaime Martins de Santiago, Paula Beatriz Silva, Ionizete Garcia da Sousa, Marcelo Valle de |
| author_facet | Santiago, Paula Beatriz Assumpção, Teresa Cristina França de Araújo, Carla Nunes de Bastos, Izabela Marques Dourado Neves, David Silva, Ionizete Garcia da Charneau, Sébastien Queiroz, Rayner Myr Lauterjung Raiol, Tainá Oliveira, João Victor de Araújo Sousa, Marcelo Valle de Calvo, Eric Ribeiro, José M. C. Santana, Jaime Martins de |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/24200/1/ARTIGO_DeepInsightSialome.pdf http://repositorio2.unb.br/jspui/bitstream/10482/24200/2/license.txt |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Santiago, Paula Beatriz Assumpção, Teresa Cristina França de Araújo, Carla Nunes de Bastos, Izabela Marques Dourado Neves, David Silva, Ionizete Garcia da Charneau, Sébastien Queiroz, Rayner Myr Lauterjung Raiol, Tainá Oliveira, João Victor de Araújo Sousa, Marcelo Valle de Calvo, Eric Ribeiro, José M. C. Santana, Jaime Martins de |
| dc.date.accessioned.fl_str_mv | 2017-08-21T15:23:53Z |
| dc.date.available.fl_str_mv | 2017-08-21T15:23:53Z |
| dc.date.issued.fl_str_mv | 2016-04-29 |
| dc.identifier.citation.fl_str_mv | SANTIAGO, Paula Beatriz et al. A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease. Plos Neglected Tropical Diseases, v. 10, n. 4, Article e0004581, 29 abr. 2016. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581>. Acesso em: 21 jun. 217. doi: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://dx.doi.org/10.1371/journal.pntd.0004581 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/24200 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Plos One |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Triatoma Chagas, Doença de Saliva Proteínas |
| dc.title.pt_BR.fl_str_mv | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Background Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immuneresponses. Methods/Principal Findings Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva.We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_1403f078f669ec926231ba055eea7ef1 |
| identifier_str_mv | SANTIAGO, Paula Beatriz et al. A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease. Plos Neglected Tropical Diseases, v. 10, n. 4, Article e0004581, 29 abr. 2016. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581>. Acesso em: 21 jun. 217. doi: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/24200 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher.none.fl_str_mv | Plos One |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Santiago, Paula BeatrizAssumpção, Teresa Cristina França deAraújo, Carla Nunes deBastos, Izabela Marques DouradoNeves, DavidSilva, Ionizete Garcia daCharneau, SébastienQueiroz, Rayner Myr LauterjungRaiol, TaináOliveira, João Victor de AraújoSousa, Marcelo Valle deCalvo, EricRibeiro, José M. C.Santana, Jaime Martins de2017-08-21T15:23:53Z2017-08-21T15:23:53Z2016-04-29SANTIAGO, Paula Beatriz et al. A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease. Plos Neglected Tropical Diseases, v. 10, n. 4, Article e0004581, 29 abr. 2016. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581>. Acesso em: 21 jun. 217. doi: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581.http://repositorio.unb.br/handle/10482/24200https://dx.doi.org/10.1371/journal.pntd.0004581Plos OneCopyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.info:eu-repo/semantics/openAccessA Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTriatomaChagas, Doença deSalivaProteínasBackground Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immuneresponses. Methods/Principal Findings Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva.We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_DeepInsightSialome.pdfARTIGO_DeepInsightSialome.pdfapplication/pdf8946675http://repositorio2.unb.br/jspui/bitstream/10482/24200/1/ARTIGO_DeepInsightSialome.pdf09d0eca234ef26bd4c42f9a72e636566MD51open accessLICENSElicense.txtlicense.txttext/plain160http://repositorio2.unb.br/jspui/bitstream/10482/24200/2/license.txtcd7f2de909e07c008880b5201378c22eMD52open access10482/242002023-05-24 20:34:35.704open accessoai:repositorio.unb.br:10482/24200U3VibWlzcz9vIGVmZXRpdmFkYSBwb3IgaW50ZWdyYW50ZSBkYSBlcXVpcGUgZG8gUmVwb3NpdD9yaW8gSW5zdGl0dWNpb25hbCBkYSBVbkIgZGUgYWNvcmRvIGNvbSBsaWNlbj9hIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:35Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease Santiago, Paula Beatriz Triatoma Chagas, Doença de Saliva Proteínas |
| status_str | publishedVersion |
| title | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| title_full | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| title_fullStr | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| title_full_unstemmed | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| title_short | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| title_sort | A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease |
| topic | Triatoma Chagas, Doença de Saliva Proteínas |
| url | http://repositorio.unb.br/handle/10482/24200 https://dx.doi.org/10.1371/journal.pntd.0004581 |