A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease
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| Glavni autor: | |
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| Datum izdanja: | 2016 |
| Daljnji autori: | , , , , , , , , , , , , |
| Format: | Article |
| Jezik: | eng |
| Izvor: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/24200 https://dx.doi.org/10.1371/journal.pntd.0004581 |
Sažetak: | Background Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immuneresponses. Methods/Principal Findings Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva.We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. |
Slični predmeti: A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease
- Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease
- Análise proteômica comparativa da saliva entre diferentes períodos após a alimentação sanguínea de Triatoma dimidiata, triatomíneo vetor da doença de Chagas
- An integrative sialomic analysis reveals molecules from Triatoma sordida (Hemiptera: Reduviidae)
- Descrição extensiva dos sialomas de Rhodnius neglectus e Triatoma dimidiata, vetores da doença de Chagas
- Chagas disease ecoepidemiology and environmental changes in northern Minas Gerais state, Brazil
- Efeito antimicrobiano e citolítico da trialisina recombinante da saliva de Triatoma infestans