Potencial de óleo de chia (Salvia hispanica) no controle do mofo cinzento em pós-colheita de morangos e de Botrytis cinerea in vitro

• Main Author: Oligini, Karine Fuschter
• Publication Date: 2016
• Format: Bachelor thesis
• Language: por
• Source: Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))
• Download full: http://repositorio.utfpr.edu.br/jspui/handle/1/10725
• topic: Essências e óleos essenciais; Morango - Cultivo; Botrytis cinerea; Essences and essential oils; Strawberries - Planting; CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE

Currently Brazil is one of the largest strawberry producer, but its production is severely limited by problems in its supply chain and post-harvest due to gray mold, which is caused by Botrytis cinerea, present in all producing regions of Brazil . The objective is through this work was to evaluate the potential of chia oil (Salvia hispanica) in the control of gray mold in post-harvest strawberries and Botrytis cinerea in vitro. Strawberry fruits have been subjected to treatment by micro, chia oil at different concentrations (0.125, 0.25, 0.5 and 1%), and the control using distilled water. After 24 hours, the fruits were inoculated with a suspension of Botrytis cinerea spores (5x10-4 spores ml-1). The design was completely randomized (CRD) with 4 replications of 20 fruits. The fruits were stored in controlled temperature of 10 ° C (± 1) and after 96 hours, there was mass loss evaluation, color, firmness, soluble solids (TSS), titratable acidity (TA), incidence and severity of mold gray. Even fruits were withdrawn sample to determine the activity of proteins related to pathogenicity (PRPs) and they phenylalanine ammonia lyase (PAL), chitinases and β-1,3glucanase. In vitro experiment, Petri [marca registrada] plates were used as experimental unit, in four replications, incorporating chia oil to PDA medium (Potato Dextrose Agar and) in the same experiment concentrations in postharvest. In the center of the card, a 3 mm disc mycelium of B. cinerea was inserted, and the plates kept in BOD for 96 hours at 10 ° C ± 1 ° C, which evaluated the mycelial growth process, which is outlined in DIC with plots subdivided in time. chia oil acted in reducing the incidence of gray mold in post-harvest strawberries cv. Real way, activating the enzyme chitinase. It showed no action on the FAL, and the β-1,3 glucanase. And did not alter the physicochemical characteristics of fruits. In vitro, showed no action on the B.cinerea pathogen. In this sense, it is concluded that the chia oil has potential gray mold control in strawberries, and that such action is related to activation of resistance induction process with the preferred route of activation of hydrolytic enzyme chitinase.