Radiação UV-C altera a coloração e o potencial antioxidante do tegumento de feijão carioca

• Autor(a) principal: Gois, Tiago Rui
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))
• Texto Completo: http://repositorio.utfpr.edu.br/jspui/handle/1/31145
• topic: Antioxidantes; Colorimetria; Feijão-comum; Fenóis; Plantas - Melhoramento genético; Antioxidants; Colorimetry; Common bean; Phenols; Plant breeding; CNPQ::CIENCIAS AGRARIAS::AGRONOMIA

Beans are a type of legume consumed in Brazil and around the world, and carioca beans are part of the Brazilian diet. However, during processing, changes in tegument colors occur, influencing the commercial reception of the grain. The chamber with UV-C light allows the simulation of accelerated aging and the observation of changes in the color of the integument. The present study aimed to characterize 25 genotypes of carioca bean recognized by simulated aging by UV-C light in terms of tegument color and antioxidant potential. Twenty-two lines and three parents were used and cultivated in the experimental area of UTFPR – Pato Branco. Genotypes were initially evaluated for yield components, and after being tolerated under UV-C light for 72 hours, they were then evaluated for color variables (in terms of L* - luminosity), antioxidant potential by ABTS, DPPH, and FRAP methods, and estimation of the total content of phenolic compounds (CFT) and flavonoids (FT). The results obtained were examined using an analysis of variance, a t-test (comparison between before and after UV-C), and the Scott and Knott mean test. For the yield components, the lines that presented the highest values were lines 252, 116, 145, 178, and 320, presenting a thousand-grain mass superior to their parents (in the same cultivation condition) or values similar to some cultivars available in the market (L252, L116, L145). The application of UV-C light resulted in a significant difference in tegument colors in most genotypes, being above the suggested value for carioca bean (L* 55) in only five lines (L116, L128, 145, L162, L202, and L178). The CFT and FT contents also showed a significant difference before and after the application of UV-C light, with a general decrease in phenolic compounds and an increase in total flavonoids. For CFT, strains 173 and 310 showed the highest values after UV-C application. For FT, strain 320 showed the highest value after UV-C application, followed by 173 (group b of means) and 310 (group c of means). For the analysis of antioxidant activity, in all three methods, there was a significant difference in the evaluation before and after UV-C, with the behavior being genotype-dependent. Lines 277 and 278 adopted the highest ABTS values after UV-C, followed by line 310 and the cultivar IPR Tangará. For the DPPH method, strains 178, 277, 278, and 320 were the ones that showed the highest values after UV-C, and for FRAP, strain 173 showed the highest value. Based on the results, it is possible to conclude that lines 173, 178, 310, and 320 presented the best result in the largest of the variable variables, being promising for their future release as a cultivar since they presented tegument with L* varying between 50.418 and 55.355 even after UV-C application, a mass of a thousand grain varying between 164.89 and 234.08 g, higher potential antioxidants by ABTS, DPPH, and FRAP methods, and higher CFT and FT values after UV-C application.