Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes

• Main Author: De Mattos, Ivanildo Luiz
• Publication Date: 2002
• Other Authors: Gorton, Lo, Ruzgas, Tautgirdas
• Format: Article
• Language: eng
• Source: Repositório Institucional da UNESP
• Download full: http://dx.doi.org/10.1016/S0956-5663(02)00185-9; http://hdl.handle.net/11449/230923
• topic: Amperometric sensor; Biosensor; Prussian Blue; Screen-printed electrodes

Gold (Au) and platinum (Pt) screen-printed electrodes were modified with Prussian Blue (PB) for the development of amperometric sensors selective for hydrogen peroxide detection. The sensors exhibited sensitivities towards H2O2 equal to 2 A M-1 cm-2 for Au and 1 A M-1 cm-2 for Pt electrodes. The sensors were also employed as the basis for construction of glucose biosensors through further modification with crystallised glucose oxidase immobilised in a Nafion membrane. In order to improve the operational stability of the modified electrodes a buffer solution containing tetrabutylammonium toluene-4-sulfonate was used. The long-term performance of the sensors and biosensors were evaluated by continuous monitoring of hydrogen peroxide and glucose solutions (50 μM and 1 mM, respectively) in the flow-injection mode for 10 h. © 2002 Elsevier Science B.V. All rights reserved.