Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus

• Main Author: Bertolini, Amanda Bezerra [UNESP]
• Publication Date: 2023
• Other Authors: Thyssen, Patrícia Jacqueline, Brandão, Paulo Eduardo, Prado, Aline Marrara, Silva, Sheila Oliveira de Souza, Mioni, Mateus de Souza Ribeiro [UNESP], de Gouvea, Fábio Lucas Rezende [UNESP], Pantoja, José Carlos de Figueiredo [UNESP], Langoni, Helio [UNESP], Guimarães, Felipe Freitas [UNESP], Joaquim, Sâmea Fernandes [UNESP], Guerra, Simony Trevizan [UNESP], Leite, Domingos da Silva, Rall, Vera Mores [UNESP], Hernandes, Rodrigo Tavanelli [UNESP], Lucheis, Simone Baldini [UNESP], Rossi, Gabriel Augusto Marques, Ribeiro, Márcio Garcia [UNESP]
• Format: Article
• Language: eng
• Source: Repositório Institucional da UNESP
• Download full: http://dx.doi.org/10.1093/jambio/lxad020; http://hdl.handle.net/11449/248443
• topic: BCoV; dairy cows; flying insects; RVA; Vector-borne diseases

AIMS: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. METHODS AND RESULTS: We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens. CONCLUSIONS: Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.