Análise histomorfométrica do processo de reparo de cavidades cirúrgicas preenchidas com osso inorgânico de origem bovina (Bonefill) em tíbia de ratos

• Main Author: Polo, Tárik Ocon Braga [UNESP]
• Publication Date: 2013
• Format: Bachelor thesis
• Language: por
• Source: Repositório Institucional da UNESP
• Download full: http://hdl.handle.net/11449/149618; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-30/000856658.pdf
• topic: Materiais biomédicos; Regeneração óssea; Aloenxertos; Implantes dentários; Biomedical materials

The purpose of this study was to evaluate qualitatively and quantitatively the process of bone repair surgical defects filled with blood clot (CS), autogenous bone (OA), or inorganic bovine bone (BB) in the tibia of adult male Wistar rats. Surgical defects were created in the right and left tibia forty animals were divided into four groups: the control group, in which the surgical defect in the right and left tibia were filled with OA and CS, respectively, the BB group, in which the defect surgery was filled with inorganic bovine bone. The animals were sacrificed after 10 and 30 days post-operative tissue block and tibia were dissected and decalcified. The samples were processed histologically and stained with hematoxylin and eosin technique (HE) and Masson trichrome and subjected to histometric analysis. The complete repair of the bone defect was observed in all groups at day 30, however, the inorganic bovine bone was not absorbed in the BB group. Histomorphometric analysis revealed a significant increase mineralized tissue in the BB group compared to the control treatment group. The inorganic bovine bone used showed biocompatibility and osteoconductive potential