Export Ready — 

Gut dysbiosis in mice genetically selected for low antibody production

Bibliographic Details
Main Author: Da Silva Santos, Ana Carolina [UNESP]
Publication Date: 2017
Other Authors: Jensen, José Ricardo, De Oliveira, Silvio Luis [UNESP], Rodrigues, Josias [UNESP]
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://dx.doi.org/10.1186/s13099-017-0193-x
http://hdl.handle.net/11449/175015
Summary: Background: Dysbiosis is linked to the cause of several human diseases, many of which having an immunity related component. This work investigated whether mice genetically selected for low or high antibody production display differences in intestinal bacterial communities, and consisted in the comparison of fecal 16SV6-V8 rDNA PCR amplicons resolved by temperature gradient gel electrophoresis (TGGE) of five each of low (LIII) and high (HIII) antibody producing mice. 16SV6 rDNA amplicons of 2 mice from each line were sequenced. Results: LIII mice were grouped in a single TGGE cluster, displayed a low α-diversity, and were distinguished by low Firmicutes/Bacteroidetes ratio. Conclusion: The results suggest that genetically driven low antibody production in mice is associated with gut dysbiosis.
id UNSP_287f83f97613f891dbe2e83e23dbdaa1
oai_identifier_str oai:repositorio.unesp.br:11449/175015
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Gut dysbiosis in mice genetically selected for low antibody productionAntibodyBacteroidetesFirmicutesMicrobiomePrevotellaceaeRuminococcaceaeBackground: Dysbiosis is linked to the cause of several human diseases, many of which having an immunity related component. This work investigated whether mice genetically selected for low or high antibody production display differences in intestinal bacterial communities, and consisted in the comparison of fecal 16SV6-V8 rDNA PCR amplicons resolved by temperature gradient gel electrophoresis (TGGE) of five each of low (LIII) and high (HIII) antibody producing mice. 16SV6 rDNA amplicons of 2 mice from each line were sequenced. Results: LIII mice were grouped in a single TGGE cluster, displayed a low α-diversity, and were distinguished by low Firmicutes/Bacteroidetes ratio. Conclusion: The results suggest that genetically driven low antibody production in mice is associated with gut dysbiosis.Department of Microbiology and Immunology Institute of Biosciences State University of São Paulo (UNESP) Campus de Rubião JuniorLaboratory of Immunogenetics Butantan Institute, Av. Dr. Vital Brazil 1500Department of Microbiology and Immunology Institute of Biosciences State University of São Paulo (UNESP) Campus de Rubião JuniorUniversidade Estadual Paulista (Unesp)Butantan InstituteDa Silva Santos, Ana Carolina [UNESP]Jensen, José RicardoDe Oliveira, Silvio Luis [UNESP]Rodrigues, Josias [UNESP]2018-12-11T17:13:51Z2018-12-11T17:13:51Z2017-08-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s13099-017-0193-xGut Pathogens, v. 9, n. 1, 2017.1757-4749http://hdl.handle.net/11449/17501510.1186/s13099-017-0193-x2-s2.0-850269046132-s2.0-85026904613.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGut Pathogens1,066info:eu-repo/semantics/openAccess2024-10-14T15:06:22Zoai:repositorio.unesp.br:11449/175015Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-14T15:06:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Gut dysbiosis in mice genetically selected for low antibody production
title Gut dysbiosis in mice genetically selected for low antibody production
spellingShingle Gut dysbiosis in mice genetically selected for low antibody production
Da Silva Santos, Ana Carolina [UNESP]
Antibody
Bacteroidetes
Firmicutes
Microbiome
Prevotellaceae
Ruminococcaceae
title_short Gut dysbiosis in mice genetically selected for low antibody production
title_full Gut dysbiosis in mice genetically selected for low antibody production
title_fullStr Gut dysbiosis in mice genetically selected for low antibody production
title_full_unstemmed Gut dysbiosis in mice genetically selected for low antibody production
title_sort Gut dysbiosis in mice genetically selected for low antibody production
author Da Silva Santos, Ana Carolina [UNESP]
author_facet Da Silva Santos, Ana Carolina [UNESP]
Jensen, José Ricardo
De Oliveira, Silvio Luis [UNESP]
Rodrigues, Josias [UNESP]
author_role author
author2 Jensen, José Ricardo
De Oliveira, Silvio Luis [UNESP]
Rodrigues, Josias [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Butantan Institute
dc.contributor.author.fl_str_mv Da Silva Santos, Ana Carolina [UNESP]
Jensen, José Ricardo
De Oliveira, Silvio Luis [UNESP]
Rodrigues, Josias [UNESP]
dc.subject.por.fl_str_mv Antibody
Bacteroidetes
Firmicutes
Microbiome
Prevotellaceae
Ruminococcaceae
topic Antibody
Bacteroidetes
Firmicutes
Microbiome
Prevotellaceae
Ruminococcaceae
description Background: Dysbiosis is linked to the cause of several human diseases, many of which having an immunity related component. This work investigated whether mice genetically selected for low or high antibody production display differences in intestinal bacterial communities, and consisted in the comparison of fecal 16SV6-V8 rDNA PCR amplicons resolved by temperature gradient gel electrophoresis (TGGE) of five each of low (LIII) and high (HIII) antibody producing mice. 16SV6 rDNA amplicons of 2 mice from each line were sequenced. Results: LIII mice were grouped in a single TGGE cluster, displayed a low α-diversity, and were distinguished by low Firmicutes/Bacteroidetes ratio. Conclusion: The results suggest that genetically driven low antibody production in mice is associated with gut dysbiosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-07
2018-12-11T17:13:51Z
2018-12-11T17:13:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s13099-017-0193-x
Gut Pathogens, v. 9, n. 1, 2017.
1757-4749
http://hdl.handle.net/11449/175015
10.1186/s13099-017-0193-x
2-s2.0-85026904613
2-s2.0-85026904613.pdf
url http://dx.doi.org/10.1186/s13099-017-0193-x
http://hdl.handle.net/11449/175015
identifier_str_mv Gut Pathogens, v. 9, n. 1, 2017.
1757-4749
10.1186/s13099-017-0193-x
2-s2.0-85026904613
2-s2.0-85026904613.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gut Pathogens
1,066
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1834484467544096768

Similar Items