Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows

Detalhes bibliográficos
Autor(a) principal: Camargos, A.
Data de Publicação: 2017
Outros Autores: Wohlres-Viana, S., Costa, I. F. [UNESP], Camargo, L. S., Ferreira, J. C. [UNESP], Ramos, A. A. [UNESP], Oba, E. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-4162-9318
http://hdl.handle.net/11449/179200
Resumo: The present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein- Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P < 0.05) but no difference (P > 0.05) between treatments was found for PTGFR and ERS1 expression. Estrogen concentrations increased progressively until day 14 (P < 0.05) and the highest OXTR expression and lowest PTGFR expression were observed on day 14 (P < 0.05) in both treatments. Serum PGFM concentrations were high throughout the experiment. In conclusion, cloprostenol administration at days two and five of postpartum seems to reduce OXTR expression in the endometrium in crossbred cows.
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spelling Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cowsESR1EstradiolProgesteronePTGFRRT-PCRThe present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein- Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P < 0.05) but no difference (P > 0.05) between treatments was found for PTGFR and ERS1 expression. Estrogen concentrations increased progressively until day 14 (P < 0.05) and the highest OXTR expression and lowest PTGFR expression were observed on day 14 (P < 0.05) in both treatments. Serum PGFM concentrations were high throughout the experiment. In conclusion, cloprostenol administration at days two and five of postpartum seems to reduce OXTR expression in the endometrium in crossbred cows.Instituto Federal Goiano - IF GoianoEmpresa Brasileira de Pesquisa Agropecuária - EmbrapaUniversidade Estadual Paulista 'Julio de Mesquita Filho' - UnespUniversidade Estadual Paulista 'Julio de Mesquita Filho' - UnespInstituto Federal Goiano - IF GoianoEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Camargos, A.Wohlres-Viana, S.Costa, I. F. [UNESP]Camargo, L. S.Ferreira, J. C. [UNESP]Ramos, A. A. [UNESP]Oba, E. [UNESP]2018-12-11T17:34:11Z2018-12-11T17:34:11Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article821-829application/pdfhttp://dx.doi.org/10.1590/1678-4162-9318Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 69, n. 4, p. 821-829, 2017.1678-41620102-0935http://hdl.handle.net/11449/17920010.1590/1678-4162-9318S0102-093520170004008212-s2.0-85029711114S0102-09352017000400821.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinaria e Zootecnia0,248info:eu-repo/semantics/openAccess2024-09-09T14:00:46Zoai:repositorio.unesp.br:11449/179200Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-03-28T15:40:30.944133Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
title Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
spellingShingle Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
Camargos, A.
ESR1
Estradiol
Progesterone
PTGFR
RT-PCR
title_short Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
title_full Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
title_fullStr Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
title_full_unstemmed Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
title_sort Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
author Camargos, A.
author_facet Camargos, A.
Wohlres-Viana, S.
Costa, I. F. [UNESP]
Camargo, L. S.
Ferreira, J. C. [UNESP]
Ramos, A. A. [UNESP]
Oba, E. [UNESP]
author_role author
author2 Wohlres-Viana, S.
Costa, I. F. [UNESP]
Camargo, L. S.
Ferreira, J. C. [UNESP]
Ramos, A. A. [UNESP]
Oba, E. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Federal Goiano - IF Goiano
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Camargos, A.
Wohlres-Viana, S.
Costa, I. F. [UNESP]
Camargo, L. S.
Ferreira, J. C. [UNESP]
Ramos, A. A. [UNESP]
Oba, E. [UNESP]
dc.subject.por.fl_str_mv ESR1
Estradiol
Progesterone
PTGFR
RT-PCR
topic ESR1
Estradiol
Progesterone
PTGFR
RT-PCR
description The present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein- Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P < 0.05) but no difference (P > 0.05) between treatments was found for PTGFR and ERS1 expression. Estrogen concentrations increased progressively until day 14 (P < 0.05) and the highest OXTR expression and lowest PTGFR expression were observed on day 14 (P < 0.05) in both treatments. Serum PGFM concentrations were high throughout the experiment. In conclusion, cloprostenol administration at days two and five of postpartum seems to reduce OXTR expression in the endometrium in crossbred cows.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T17:34:11Z
2018-12-11T17:34:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-4162-9318
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 69, n. 4, p. 821-829, 2017.
1678-4162
0102-0935
http://hdl.handle.net/11449/179200
10.1590/1678-4162-9318
S0102-09352017000400821
2-s2.0-85029711114
S0102-09352017000400821.pdf
url http://dx.doi.org/10.1590/1678-4162-9318
http://hdl.handle.net/11449/179200
identifier_str_mv Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 69, n. 4, p. 821-829, 2017.
1678-4162
0102-0935
10.1590/1678-4162-9318
S0102-09352017000400821
2-s2.0-85029711114
S0102-09352017000400821.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinaria e Zootecnia
0,248
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 821-829
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1834483087674703872

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