Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Brazilian Journal of Global Health | Resumo: | OBJECTIVE: The objective of this study was to report the occurrence of Ehrlichia canis, Babesia canis vogeli and Rangelia vitalli, in ticks collected from dogs residing in the city of Itu, SP, Brazil. METHODS: DNA extraction from 200 tick samples was performed using the “PureLink Genomic DNA Kit” (Invitrogen®) extraction kit according to the manufacturer's instructions and real-time PCR was performed for the detection of Ehrlichia canis, Rangelia vitalli, Babesia canis vogeli. RESULTS: A total of 200 ticks were tested, 1/200 (0.5%) of the species of the genus Amblyomma aureolatum and 199/200 (99.5%) of Rhipicephalus sanguineus. The results show a positivity rate of occurrence only in R. sanguineus, being 0.5% (1/200) for E. canis; 41% (82/100) for B.c. vogeli. No ticks were positive for R. vitalli. CONCLUSION: Detection of B. canis vogeli and E. canis in ticks collected from dogs residing in the municipality of Itu, in the State of São Paulo, Brazil; show the dispersion of these pathogens in the country and; the role of R. sanguineus s.l. as a vector of these pathogens cannot be neglected. Descriptors: Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks. |
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oai:periodicos.unisa.br:article/368 |
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Brazilian Journal of Global Health |
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Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SPEhrlichia canisBabesia canis vogeliRangelia vitallicarrapatosEhrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks.OBJECTIVE: The objective of this study was to report the occurrence of Ehrlichia canis, Babesia canis vogeli and Rangelia vitalli, in ticks collected from dogs residing in the city of Itu, SP, Brazil. METHODS: DNA extraction from 200 tick samples was performed using the “PureLink Genomic DNA Kit” (Invitrogen®) extraction kit according to the manufacturer's instructions and real-time PCR was performed for the detection of Ehrlichia canis, Rangelia vitalli, Babesia canis vogeli. RESULTS: A total of 200 ticks were tested, 1/200 (0.5%) of the species of the genus Amblyomma aureolatum and 199/200 (99.5%) of Rhipicephalus sanguineus. The results show a positivity rate of occurrence only in R. sanguineus, being 0.5% (1/200) for E. canis; 41% (82/100) for B.c. vogeli. No ticks were positive for R. vitalli. CONCLUSION: Detection of B. canis vogeli and E. canis in ticks collected from dogs residing in the municipality of Itu, in the State of São Paulo, Brazil; show the dispersion of these pathogens in the country and; the role of R. sanguineus s.l. as a vector of these pathogens cannot be neglected. Descriptors: Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks.OBJETIVO: Objetivou-se nesse estudo relatar a ocorrência de Ehrlichia canis, Babesia canis vogeli e Rangelia vitalli, em carrapatos coletados em cães residentes no município de Itu_São Paulo/SP, Brasil. MÉTODOS: Foi realizada a extração de DNA de 200 amostras de carrapatos com o “kit” de extração “PureLink Genomic DNA Kit” (Invitrogen®) e realizada a PCR em tempo real para a detecção de Ehrlichia canis, Rangelia vitalli, Babesia canis vogeli. RESULTADOS: Foram testados 200 carrapatos, sendo 1/200 (0,5%) da espécie do gênero Amblyomma aureolatum e 199/200 (99,5%) de Rhipicephalus sanguineus. Os resultados mostram uma taxa de ocorrência de positividade apenas em R. sanguineus, sendo de 0,5% (1/200) para E. canis; 41% (82/100) para B. c. vogeli. Nenhum carrapato foi positivo para R. vitalli. CONCLUSÃO: A detecção de B. canis vogeli e E. canis em carrapatos coletados em cães residentes no município de Itu, no Estado de São Paulo, Brasil; mostram a dispersão destes agentes patogênicos no país e o papel do R. sanguineus s.l. como vetor destes patógenos não pode ser negligenciado. DESCRITORES: Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, Carrapatos. ABSTRACT OBJECTIVE: The aim of this study was to report the occurrence of Ehrlichia canis, Babesia canis vogeli and Rangelia vitalli in ticks collected from dogs living in the city of Itu_São Paulo/SP, Brazil. METHODS: DNA was extracted from 200 tick samples using the extraction kit PureLink Genomic DNA Kit (Invitrogen®) and real-time PCR was performed for the detection of Ehrlichia canis, Rangelia vitalli and Babesia canis vogeli. RESULTS: We tested 200 ticks, 1/200 (0.5%) of the genus Amblyomma aureolatum and 199/200 (99.5%) of Rhipicephalus sanguineus. The results show an occurrence rate of positivity only in R. sanguineus, being 0.5% (1/200) for E. canis; 41% (82/100) for B. c. vogeli. No ticks were positive for R. vitalli. CONCLUSION: The detection of B. canis vogeli and E. canis in ticks collected from dogs living in the municipality of Itu, São Paulo State, Brazil; show the dispersion of these pathogens in the country and the role of R. sanguineus s.l. as a vector of these pathogens cannot be neglected. DESCRIPTORS: Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, Ticks. Universidade Santo Amaro (UNISA)2022-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdf//periodicos.unisa.br/index.php/saudeglobal/article/view/36810.56242/globalhealth;2022;2;8;20-22 Brazilian Journal of Global Health; Vol. 2 No. 8 (2022): Brazilian Journal of Global Health (ISSN 2763-5368).; 20-22Brazilian Journal of Global Health; v. 2 n. 8 (2022): Brazilian Journal of Global Health (ISSN 2763-5368).; 20-222763-5368reponame:Brazilian Journal of Global Healthinstname:Universidade Santo Amaro (UNISA)instacron:UNISAporeng//periodicos.unisa.br/index.php/saudeglobal/article/view/368/424//periodicos.unisa.br/index.php/saudeglobal/article/view/368/425Copyright (c) 2022 Brazilian Journal of Global Healthinfo:eu-repo/semantics/openAccessCarvalho, TâniaAndrade, PaulaMoraes Filho, Jonas2023-09-14T20:02:36Zoai:periodicos.unisa.br:article/368Revistahttps://periodicos.unisa.br/index.php/saudeglobal/indexPRIhttps://periodicos.unisa.br/index.php/saudeglobal/oairbsg@unisa.br || rbsg.pcolombo@unisa.br2763-53682763-5368opendoar:2023-09-14T20:02:36Brazilian Journal of Global Health - Universidade Santo Amaro (UNISA)false |
dc.title.none.fl_str_mv |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
title |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
spellingShingle |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP Carvalho, Tânia Ehrlichia canis Babesia canis vogeli Rangelia vitalli carrapatos Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks. |
title_short |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
title_full |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
title_fullStr |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
title_full_unstemmed |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
title_sort |
Research of hemoparasites in ticks collected from dogs resident in the municipality of Itu, São Paulo, SP |
author |
Carvalho, Tânia |
author_facet |
Carvalho, Tânia Andrade, Paula Moraes Filho, Jonas |
author_role |
author |
author2 |
Andrade, Paula Moraes Filho, Jonas |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Carvalho, Tânia Andrade, Paula Moraes Filho, Jonas |
dc.subject.por.fl_str_mv |
Ehrlichia canis Babesia canis vogeli Rangelia vitalli carrapatos Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks. |
topic |
Ehrlichia canis Babesia canis vogeli Rangelia vitalli carrapatos Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks. |
description |
OBJECTIVE: The objective of this study was to report the occurrence of Ehrlichia canis, Babesia canis vogeli and Rangelia vitalli, in ticks collected from dogs residing in the city of Itu, SP, Brazil. METHODS: DNA extraction from 200 tick samples was performed using the “PureLink Genomic DNA Kit” (Invitrogen®) extraction kit according to the manufacturer's instructions and real-time PCR was performed for the detection of Ehrlichia canis, Rangelia vitalli, Babesia canis vogeli. RESULTS: A total of 200 ticks were tested, 1/200 (0.5%) of the species of the genus Amblyomma aureolatum and 199/200 (99.5%) of Rhipicephalus sanguineus. The results show a positivity rate of occurrence only in R. sanguineus, being 0.5% (1/200) for E. canis; 41% (82/100) for B.c. vogeli. No ticks were positive for R. vitalli. CONCLUSION: Detection of B. canis vogeli and E. canis in ticks collected from dogs residing in the municipality of Itu, in the State of São Paulo, Brazil; show the dispersion of these pathogens in the country and; the role of R. sanguineus s.l. as a vector of these pathogens cannot be neglected. Descriptors: Ehrlichia canis, Babesia canis vogeli, Rangelia vitalli, ticks. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
//periodicos.unisa.br/index.php/saudeglobal/article/view/368 10.56242/globalhealth;2022;2;8;20-22 |
identifier_str_mv |
//periodicos.unisa.br/index.php/saudeglobal/article/view/368 10.56242/globalhealth;2022;2;8;20-22 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
//periodicos.unisa.br/index.php/saudeglobal/article/view/368/424 //periodicos.unisa.br/index.php/saudeglobal/article/view/368/425 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Global Health info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Global Health |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Santo Amaro (UNISA) |
publisher.none.fl_str_mv |
Universidade Santo Amaro (UNISA) |
dc.source.none.fl_str_mv |
Brazilian Journal of Global Health; Vol. 2 No. 8 (2022): Brazilian Journal of Global Health (ISSN 2763-5368).; 20-22 Brazilian Journal of Global Health; v. 2 n. 8 (2022): Brazilian Journal of Global Health (ISSN 2763-5368).; 20-22 2763-5368 reponame:Brazilian Journal of Global Health instname:Universidade Santo Amaro (UNISA) instacron:UNISA |
instname_str |
Universidade Santo Amaro (UNISA) |
instacron_str |
UNISA |
institution |
UNISA |
reponame_str |
Brazilian Journal of Global Health |
collection |
Brazilian Journal of Global Health |
repository.name.fl_str_mv |
Brazilian Journal of Global Health - Universidade Santo Amaro (UNISA) |
repository.mail.fl_str_mv |
rbsg@unisa.br || rbsg.pcolombo@unisa.br |
_version_ |
1839270050395062272 |