Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines

Bibliographic Details
Main Author: Fajardo,Thor Vinícius Martins
Publication Date: 2017
Other Authors: Vanni,Marcos Fernando, Nickel,Osmar
Format: Article
Language: eng
Source: Ciência Rural
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600155
Summary: ABSTRACT: The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may contribute to improve virus diagnosis by accurately quantifying virus titre variations in grapevines.
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spelling Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevinesRT-qPCRGRSPaVGVAGVDGLRaV-3 and -4ABSTRACT: The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may contribute to improve virus diagnosis by accurately quantifying virus titre variations in grapevines.Universidade Federal de Santa Maria2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600155Ciência Rural v.47 n.6 2017reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20161063info:eu-repo/semantics/openAccessFajardo,Thor Vinícius MartinsVanni,Marcos FernandoNickel,Osmareng2017-05-02T00:00:00ZRevista
dc.title.none.fl_str_mv Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
title Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
spellingShingle Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
Fajardo,Thor Vinícius Martins
RT-qPCR
GRSPaV
GVA
GVD
GLRaV-3 and -4
title_short Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
title_full Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
title_fullStr Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
title_full_unstemmed Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
title_sort Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
author Fajardo,Thor Vinícius Martins
author_facet Fajardo,Thor Vinícius Martins
Vanni,Marcos Fernando
Nickel,Osmar
author_role author
author2 Vanni,Marcos Fernando
Nickel,Osmar
author2_role author
author
dc.contributor.author.fl_str_mv Fajardo,Thor Vinícius Martins
Vanni,Marcos Fernando
Nickel,Osmar
dc.subject.por.fl_str_mv RT-qPCR
GRSPaV
GVA
GVD
GLRaV-3 and -4
topic RT-qPCR
GRSPaV
GVA
GVD
GLRaV-3 and -4
description ABSTRACT: The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may contribute to improve virus diagnosis by accurately quantifying virus titre variations in grapevines.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600155
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20161063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.47 n.6 2017
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140551326760960

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