Identificação e caracterização funcional de genes virais relacionados com a papilomatose bovina

• Autor(a) principal: Fernandes, Adriana de Oliveira
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/11244
• topic: Biologia; Ensino de biologia; Papilomavírus bovino; RNA-seq; Coinfecção; Genes; Bovine papillomavirus; RNA-seq; Functional annotation; Coinfection; CIENCIAS BIOLOGICAS

Bovine papillomavirus (BPV) is the virus that causes the infectious-contagious disease known as papillomatosis, which mainly affects cattle. The disease is known to form benign warts in epithelial tissues, however, depending on the conditions, such formations may become cancerous. It is a disease of worldwide distribution and it causes serious damage, especially to Brazil's herds, as it can lead to the loss of hundreds of cattle in a country where meat and milk production is at the heart of the agricultural and livestock economy. It is known that the same animal may be affected not only by BPV, but also by other microorganisms, and this may influence the evolution of the disease. Thus, this work seeks to identify and characterize the biological functions and processes of viral genes expressed in papillomatous lesions. For this, the RNA-seq methodology was used to sequence messenger RNA from six young animals (bovine), three infected and three non-infected by BPV. With the use of the Galaxy platform, those non-host sequences were separated, and the viral genes expressed in the lesions were identified with the BLASTn program. The functional annotation of the genes of these viruses was done with the databases of Gene Ontology, UniProtKB, KEGG and ViruSite, as well as searches in scientific articles. In total eighteen viral families were found, eight of them being of clinical relevance, with several genes related to oncogenic progression, immune system suppression and cell proliferation. Due to the lack of annotated data for most genes, more efforts are required from the academic community to organize the functional information of viral genes, facilitating annotation studies. However, this is the first study to investigate the possible influence of other viral genes on the development of papillomatous lesions as a cofactor associated with BPV infection.