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Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium

Bibliographic Details
Main Author: Oliveira, Evaleide Diniz de
Publication Date: 2005
Other Authors: Leite, Thais Serafim, Silva, Bagnolia Araujo da, Conde Garcia, Eduardo Antonio
Format: Article
Language: eng
Source: Repositório Institucional da UFS
Download full: https://ri.ufs.br/jspui/handle/riufs/20406
Summary: Ribeirão Preto
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spelling Oliveira, Evaleide Diniz deLeite, Thais SerafimSilva, Bagnolia Araujo daConde Garcia, Eduardo Antonio2024-11-08T12:27:58Z2024-11-08T12:27:58Z2005OLIVEIRA, E. D. Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, n. 1, p. 111-118, jan. 2005. Disponível em: https://www.scielo.br/j/bjmbr/a/rj4kMmfWBWvkjdYFcwJyRZR/?format=pdf&lang=en. Acesso em: 8 nov. 2024.0100-879X1414-431Xhttps://ri.ufs.br/jspui/handle/riufs/20406Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchCitrus sinensisContractilityHeartMyocardiumWoodworth phenomenonCoraçãoMiocárdioInotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atriuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRibeirão PretoThe objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of Citrus sinensis (L.) Osb. on mammalian myocardium. Crude leaf extracts have been used in folk medicine to treat neurological disorders. Some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. This motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g) and surprisingly we observed inotropic depression instead of an increase in force. The maximum effect of the crude extract was 79.4 ± 8.1% of the control force amplitude (N = 5 hearts, 10 trials, 27 ± 0.1ºC, stimulus: 2 Hz, 400 V, 0.5 ms). The EC50 for crude, ethanol, acetic, aqueous, and acetone extracts was 300, 300, 600, 1000, and 140 µg/ml, respectively, with a Hill constant of 1.8, 2.0, 2.5, 2.0, and 1.4, respectively. Blockade of cholinergic, beta-adrenergic, or opioid membrane receptors with 1.5 µM atropine sulfate, 1 µM propranolol, and 10 µM naloxone, respectively, did not change the effect of the crude extract. The acetone extract abolished the Bowditch positive staircase phenomenon (N = 5 hearts, 10 trials, 27 ± 0.1ºC), suggesting a possible reduction of the calcium inward current, and also promoted the so-called Woodworth phenomenon. The effect was concentration-dependent and indicated the existence of another inhibitory contractile mechanism such as the simultaneous activation of some of the membrane potassium channels reducing the myocardial action potential duration and further decreasing the cellular calcium entry.reponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/20406/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALInotropicEffectCitrusSinensis.pdfInotropicEffectCitrusSinensis.pdfapplication/pdf646884https://ri.ufs.br/jspui/bitstream/riufs/20406/2/InotropicEffectCitrusSinensis.pdf557702778fb27788dd87fc9f11b7a6ecMD52TEXTInotropicEffectCitrusSinensis.pdf.txtInotropicEffectCitrusSinensis.pdf.txtExtracted texttext/plain29065https://ri.ufs.br/jspui/bitstream/riufs/20406/3/InotropicEffectCitrusSinensis.pdf.txtb45968b75691444fb1ca2ec2f8fdd906MD53THUMBNAILInotropicEffectCitrusSinensis.pdf.jpgInotropicEffectCitrusSinensis.pdf.jpgGenerated Thumbnailimage/jpeg1612https://ri.ufs.br/jspui/bitstream/riufs/20406/4/InotropicEffectCitrusSinensis.pdf.jpg9a7018243b8d33ae4d75aa6f21d78d8fMD54riufs/204062024-12-05 17:30:46.157oai:oai:ri.ufs.br:repo_01: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2024-12-05T20:30:46Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
title Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
spellingShingle Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
Oliveira, Evaleide Diniz de
Citrus sinensis
Contractility
Heart
Myocardium
Woodworth phenomenon
Coração
Miocárdio
title_short Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
title_full Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
title_fullStr Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
title_full_unstemmed Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
title_sort Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium
author Oliveira, Evaleide Diniz de
author_facet Oliveira, Evaleide Diniz de
Leite, Thais Serafim
Silva, Bagnolia Araujo da
Conde Garcia, Eduardo Antonio
author_role author
author2 Leite, Thais Serafim
Silva, Bagnolia Araujo da
Conde Garcia, Eduardo Antonio
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Evaleide Diniz de
Leite, Thais Serafim
Silva, Bagnolia Araujo da
Conde Garcia, Eduardo Antonio
dc.subject.por.fl_str_mv Citrus sinensis
Contractility
Heart
Myocardium
Woodworth phenomenon
Coração
Miocárdio
topic Citrus sinensis
Contractility
Heart
Myocardium
Woodworth phenomenon
Coração
Miocárdio
description Ribeirão Preto
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2024-11-08T12:27:58Z
dc.date.available.fl_str_mv 2024-11-08T12:27:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, E. D. Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, n. 1, p. 111-118, jan. 2005. Disponível em: https://www.scielo.br/j/bjmbr/a/rj4kMmfWBWvkjdYFcwJyRZR/?format=pdf&lang=en. Acesso em: 8 nov. 2024.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/jspui/handle/riufs/20406
dc.identifier.issn.none.fl_str_mv 0100-879X
1414-431X
dc.identifier.license.pt_BR.fl_str_mv Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)
identifier_str_mv OLIVEIRA, E. D. Inotropic effect of Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, n. 1, p. 111-118, jan. 2005. Disponível em: https://www.scielo.br/j/bjmbr/a/rj4kMmfWBWvkjdYFcwJyRZR/?format=pdf&lang=en. Acesso em: 8 nov. 2024.
0100-879X
1414-431X
Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)
url https://ri.ufs.br/jspui/handle/riufs/20406
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
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