Efeito da prostaglandina e anti-inflamatório não esteroidal no processo ovulatório em bovinos

• Autor(a) principal: D'Ávila, Camila Amaral
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFPel - Guaiaca
• Texto Completo: http://guaiaca.ufpel.edu.br/handle/prefix/7931
• topic: Prostaglandina; Ovulação; Luteinização; Vascularização; Prostaglandin; Ovulation; Luteinization; Vascularization; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL

The search for better reproductive outcomes in current production systems is directly linked to the economic return to the producer. In this way, maximizing production is a very important factor. Allied to this, there is a great current concern regarding the hormonal residues used in reproduction protocols, such as esters of estradiol. Firstly, in article 1 we aimed to review the main hormones used as ovulation inducers in fixedtime artificial insemination protocols. Prostaglandin, which is widely used for the purpose of luteolysis, has shown positive effects on ovulation in bovine females and may be an alternative to estradiol esters already banned in some countries. Subsequently and based on the above, the present study aimed to evaluate 1) the effect of intramuscular prostaglandin F2α (im) during the ovulatory process, compared to GnRH and 2) if PGF treatment is able to reverse the inhibitory effects of a nonsteroidal anti-inflammatory drug (NSAID) in the ovulatory cascade induced by GnRH, testing the hypothesis that the im application of prostaglandin F2α alters the ovulatory process in bovines affecting luteinization and vascularization in the follicle. Cows (n = 19) had the follicular wave synchronized with estradiol benzoate (D0), intravaginal progesterone device (DIVP4; 9 days) and application of PGF (241 μg; i.m.) at D8. In experiment 1, after 12 h of DIVP4 withdrawal, the cows were allocated into three groups: control: saline solution 0.9% i.m (n = 6); GnRH: 100μg gonadorelin acetate i.m (n = 7); and PGF: 500μg sodium cloprostenol i.m. (n = 6). The follicles were aspirated 24 h after the treatments, there was no significant difference (P> 0.05) in the concentrations of P4 and E2 in the follicular fluid (FF), but E2 levels were lower in the GnRH group (P <0.05 ). In experiment 2, GnRH (gonadorelin acetate, 100μg i.m.) was applied after removal of DIVP4 (time 0) in all animals. Immediately after, the cows were allocated in three groups: control: saline solution 0.9% i.m (n = 6); NSAID: 2.2mg / kg i.m. of flunixin meglumine (FM) 17 h after GnRH (n = 6); and AINE + PGF group, 2.2mg / kg i.m. of FM 17h after application of GnRH and 25mg i.m. of PGF 23h after GnRH (n = 6). The AINE + PGF group had a lower concentration of P4 in the FF and less vascularization of the follicular wall (P <0.05) 24h after GnRH. There was no significant difference in intrafollicular E2 levels. It is concluded that the i.m application of PGF does not induce the same effects observed on teroidogenesis after GnRH treatment.