Pós-condicionamento com L-alanil-glutamina na isquemia e reperfusão cerebral em ratos

• Main Author: Almeida, Eduardo Bacelar
• Publication Date: 2016
• Format: Master thesis
• Language: por
• Source: Repositório Institucional da Universidade Federal do Ceará (UFC)
• dARK ID: ark:/83112/001300002pwmw
• Download full: http://www.repositorio.ufc.br/handle/riufc/25153
• topic: Isquemia Encefálica; Glutamina; Isquemia; Reperfusão

INTRODUCTION AND OBJECTIVE: Recent studies have shown the effectiveness of L-alanyl-glutamine (Aln-Gln) in protection of ischemia and reperfusion states in various organs. The aim of this study was to evaluate Aln-Gln post conditioning effects in brain ischemia and reperfusion injury in an experimental model of brain ischemia and reperfusion in rats. METHODS: A sample of 72 rats (Wistar) was divided into 06 groups. Each group had 12 animals, 6 animals provided hippocampal samples for mass spectrometry analysis and 6 animals samples for histological analysis. 02 groups carried cervicotomy (Sham 1h and 24h); 02 groups received saline and ischemia/ reperfusion injury (SS + I/R 1h and 24h), and 02 groups received L-Alanyl-Glutamine and ischemia/reperfusion injury (GLUT + I/R 1h and 24h). RESULTS: The histology (H & E) of hippocampal CA1 area revealed a significant increase (p <0.05) of eosinophilic neurons in saline + ischemia and reperfusion group (SS + I/R) compared to the control group (SHAM) after 1 hour (p = 0.002) and 24h (p = 0.01). When compared (SS + I/R) and (GLUT + I/R) groups a significant decrease (p <0.05) of percentage of eosinophilic neurons was observed in GLUT + I / R, with 1h (p = 0.01) and 24 hours of reperfusion (p = 0.001). In the hippocampal spectrometry’s analysis there was a statistically significant difference between the groups lipid profile (p <0.05). A significant difference in relative intensity of lipids and lipid profile from Sham groups when compared to SS + I/R group (p <0.05) and GLUT+ I/R when compared to SS+ I/R group (p <0.05). CONCLUSION: The postconditioning with L-alanyl-glutamine had neuroprotective effect in the hippocampus of this experimental model, indicated by the number of red neurons. The relative intensity of the hippocampus lipids in the analysis by mass spectrometry is altered in ischemic and reperfusion injury, and is different from postconditioned animals with L-alanyl-glutamine