Effects of chlorine on cells of Vibrio cholerae

• Main Author: Sousa, Oscarina Viana de
• Publication Date: 2005
• Other Authors: Vieira, Regine Helena Silva dos Fernandes, Patel, T. R., Mesquita, V. P.
• Format: Article
• Language: eng
• Source: Repositório Institucional da Universidade Federal do Ceará (UFC)
• dARK ID: ark:/83112/00130000167g1
• Download full: http://www.repositorio.ufc.br/handle/riufc/73987
• topic: Species - Vibrio cholerae; Colera; Chlorine solution; Espécie - Vibrio cholerae; Cólera; Solução clorina

Since the sixth pandemic outbreak of cholera, chlorine had been used as the ¢rst line of defense. The objective of this investigation was to determine the bactericidal concentration of chlorine against Vibrio cholerae in arti¢cially-contaminated samples of shrimps and water. Cells of V. cholerae were exposed to varying concentrations (5^10 ppm) of chlorine in solution for 5 min. Arti¢cially-contaminated shrimp (Xiphoenaeus kroyer) samples were also dipped for 5 min in chlorine solutions (10 ppm).The results strongly indicate that 8 ppm chlorine was e¡ective in killing viable cells from pure cultures. Isolates of this organism were recovered from carapace, head and tail parts of the crustaceans. V. cholerae strains re-isolated from carapace/head samples seemed to be more resistant than those from tail meat when exposed to10 ppm chlorine solution for 5 min