Cytogenotoxic and oxidative status evaluation of Morinda citrifolia

• Main Author: Moraes, Germano Pinho de
• Publication Date: 2016
• Other Authors: Alencar, Marcus Vinícius Oliveira Barros de, Islam, Torequl, Araújo, Lidiane da Silva, Sobral, André Luiz Pinho, Machado, Kátia da Conceição, Aguiar, Rai Pablo Sousa de, Gomes Júnior, Antonio Luiz, Corrêa, Dione, Paz, Márcia Fernanda Correia Jardim, Ferreira, Paulo Michel Pinheiro, Melo-Cavalcante, Ana Amélia de Carvalho, Ferraz, Alexandre, Grivicich, Ivana, Picada, Jaqueline Nascimento
• Format: Article
• Language: eng
• Source: Repositório Institucional da Universidade Federal do Ceará (UFC)
• dARK ID: ark:/83112/0013000026hqk
• Download full: http://www.repositorio.ufc.br/handle/riufc/18783
• topic: Morinda; Sarcoma 180; Saccharomyces cerevisiae

Cancer is one of the most leading causes of death worldwide. Morinda citrifolia was reported to have antitumor effects. Cisplatin (CDDP), Doxorubicin (DOX) and Cyclophosphamid (CPA) are the known effective chemotherapeutics, despite of having several side effects. This study evaluated antitumoral and oxidative effects of the aqueous extract of the fruit of M. critrifolia (AEMC) (15, 30, 60 and 120 µg/mL) in comparision to CDDP (1 and 5 μg/mL), CPA (20 μg/mL), DOX (2 μg/mL) and CPA + DOX (20:2 μg/mL) in Sarcoma 180 cells and Saccharomyces cerevisiae, respectively. Cytogenetic damage and DNA fragmentation were evaluated with cytokinesis-block micronucleus assay and comet assay, respectively. In addition, S. cerevisiae strains were used in oxidative damage evaluation. AEMC induced cytogenetic damage with the increased formation of micronuclei, nuclear buds and nucleoplasmic bridges compared to the antineoplastics tested. AEMC at 120 µg/ mL induced significant (p<0.05) DNA damage in Sarcoma 180 cells similar to the CDDP, as well as oxidative damage in S. cerevisiae strain deficient in mitochondrial superoxide dismutase (Sod2∆) and cytosolic catalase (Cat1∆). The bioactive compounds present in AEMC such as gallic, caffeic, chlorogenic, ellagic acid and rutin might be responsible for AEMC’s antitumoral activity.