A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes

Bibliographic Details
Main Author: Almeida, Paulo Fernando de
Publication Date: 2000
Other Authors: Almeida, Rogeria Comastri de Castro
Format: Article
Language: eng
Source: Repositório Institucional da UFBA
Download full: http://www.repositorio.ufba.br/ri/handle/ri/7875
Summary: Texto completo: acesso restrito. p. 97–101
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spelling Almeida, Paulo Fernando deAlmeida, Rogeria Comastri de CastroAlmeida, Paulo Fernando deAlmeida, Rogeria Comastri de Castro2013-01-16T11:36:34Z20000956-7135http://www.repositorio.ufba.br/ri/handle/ri/7875v. 11, n. 2Texto completo: acesso restrito. p. 97–101Polymerase chain reaction is a powerful technique for detection of pathogens in foods. It is a rapid procedure with both sensitivity and specificity for quick detection and identification of specific pathogenic bacteria from different sources. Listeria monocytogenes detection methods based on PCR amplification of the iap, prfA and hly gene sequences have been reported. The present study undertakes the development of an alternative PCR method using the inl gene sequences as a target to detect pathogenic L. monocytogenes. The presence of a unique and specific DNA amplification fragment of 760 bp for the intragenic repeats B of the inlA gene in all strains of L. monocytogenes as compared to none in other Listeria and unrelated Gram positive and Gram negative species confirms that this procedure is an alternative PCR protocol for detection of L. monocytogenes.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2013-01-16T11:36:34Z No. of bitstreams: 1 Almeida.pdf: 111230 bytes, checksum: 34de53acf406e0d623fc431cae17d734 (MD5)Made available in DSpace on 2013-01-16T11:36:34Z (GMT). No. of bitstreams: 1 Almeida.pdf: 111230 bytes, checksum: 34de53acf406e0d623fc431cae17d734 (MD5) Previous issue date: 2000http://dx.doi.org/10.1016/S0956-7135(99)00067-5reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPCRListeria monocytogenesInternalinA PCR protocol using inl gene as a target for specific detection of Listeria monocytogenesFood Controlinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7875/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINALAlmeida.pdfAlmeida.pdfapplication/pdf111230https://repositorio.ufba.br/bitstream/ri/7875/1/Almeida.pdf34de53acf406e0d623fc431cae17d734MD51TEXTAlmeida.pdf.txtAlmeida.pdf.txtExtracted texttext/plain21376https://repositorio.ufba.br/bitstream/ri/7875/3/Almeida.pdf.txt124029f157227b86dc8b9c53a3bfd659MD53ri/78752022-08-08 13:17:26.854oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestrepositorio@ufba.bropendoar:19322022-08-08T16:17:26Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
dc.title.alternative.pt_BR.fl_str_mv Food Control
title A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
spellingShingle A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
Almeida, Paulo Fernando de
PCR
Listeria monocytogenes
Internalin
title_short A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
title_full A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
title_fullStr A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
title_full_unstemmed A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
title_sort A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes
author Almeida, Paulo Fernando de
author_facet Almeida, Paulo Fernando de
Almeida, Rogeria Comastri de Castro
author_role author
author2 Almeida, Rogeria Comastri de Castro
author2_role author
dc.contributor.author.fl_str_mv Almeida, Paulo Fernando de
Almeida, Rogeria Comastri de Castro
Almeida, Paulo Fernando de
Almeida, Rogeria Comastri de Castro
dc.subject.por.fl_str_mv PCR
Listeria monocytogenes
Internalin
topic PCR
Listeria monocytogenes
Internalin
description Texto completo: acesso restrito. p. 97–101
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2013-01-16T11:36:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7875
dc.identifier.issn.none.fl_str_mv 0956-7135
dc.identifier.number.pt_BR.fl_str_mv v. 11, n. 2
identifier_str_mv 0956-7135
v. 11, n. 2
url http://www.repositorio.ufba.br/ri/handle/ri/7875
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/S0956-7135(99)00067-5
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/7875/2/license.txt
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https://repositorio.ufba.br/bitstream/ri/7875/3/Almeida.pdf.txt
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