Effects of lllt on the proliferation of hep2 cells: study in vitro

Bibliographic Details
Main Author: Pinheiro, Antonio Luiz Barbosa
Publication Date: 2000
Other Authors: Nascimento, Silene Carneiro do, Viera, Alessandro Leonardo de Barros, Rolim, AluIzio Barros, Silva, Pedro Soriano da, Brugnera Júnior, Aldo
Format: Article
Language: eng
Source: Repositório Institucional da UFBA
Download full: http://www.repositorio.ufba.br/ri/handle/ri/12805
Summary: p. 75-81
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spelling Pinheiro, Antonio Luiz BarbosaNascimento, Silene Carneiro doViera, Alessandro Leonardo de BarrosRolim, AluIzio BarrosSilva, Pedro Soriano daBrugnera Júnior, AldoPinheiro, Antonio Luiz BarbosaNascimento, Silene Carneiro doViera, Alessandro Leonardo de BarrosRolim, AluIzio BarrosSilva, Pedro Soriano daBrugnera Júnior, Aldo2013-09-03T12:01:38Z2000http://www.repositorio.ufba.br/ri/handle/ri/12805v. 3910p. 75-81LLLT has been used successfully in biomedicine and some of the results are thought to be related to cell proliferation. The effects of LLLT on cell proliferation is debatable because different studies found either increase or decrease on proliferation of cell cultures. Cell culture is an excellent method to assess both effects and dose of treatment. The aim of this study was to assess the effect off 635 and 670nm laser irradiation of H.Ep.2 cells In Vitro using MTT. The cells were obtained from SCC of the larynx were routinely processed from defrost to the experimental condition. Twenty four hours after transplantation the cells were irradiated with doses ranging from 0.03 a O.6J/cm2 during seven days. The results showed that 635nm laser light does not stimulate significantly the proliferation of H.Ep.2 cells in doses ranging from O.04J/cm2 to O.488J/cm2. However 670nm laser irradiation resulted in an increased cell proliferation when compared to both control and 635nm irradiated cells. It is concluded that irradiation of H.Ep.2 cells with 670nm laser results in an increased cell proliferation; best observation of cell proliferation was found on 670nm laser irradiated cultures exposed from doses of O.075J1cm2; both dose and wavelengths are factors which may affect cell proliferation of H.Ep.2 cells; doses of 635nm laser light up to O.48J/cm2 did not interfere with H.Ep.2 cell proliferation; and that the MTT was effective on the detection ofH.Ep.2 cells proliferation on both irradiated and non-irradiated cell cultures.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-30T19:45:17Z No. of bitstreams: 1 88888888888888888888.pdf: 203429 bytes, checksum: 0618e27d0a6ff107ced54fe4d2edbe00 (MD5)Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-09-03T12:01:38Z (GMT) No. of bitstreams: 1 88888888888888888888.pdf: 203429 bytes, checksum: 0618e27d0a6ff107ced54fe4d2edbe00 (MD5)Made available in DSpace on 2013-09-03T12:01:38Z (GMT). No. of bitstreams: 1 88888888888888888888.pdf: 203429 bytes, checksum: 0618e27d0a6ff107ced54fe4d2edbe00 (MD5) Previous issue date: 2000SalvadorProceedings of SPIECell proliferationHazardsStimulatory effectEffects of lllt on the proliferation of hep2 cells: study in vitroProceedings of SPIEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01engreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINAL88888888888888888888.pdf88888888888888888888.pdfapplication/pdf203429https://repositorio.ufba.br/bitstream/ri/12805/1/88888888888888888888.pdf0618e27d0a6ff107ced54fe4d2edbe00MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12805/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT88888888888888888888.pdf.txt88888888888888888888.pdf.txtExtracted texttext/plain15615https://repositorio.ufba.br/bitstream/ri/12805/3/88888888888888888888.pdf.txtdd86cf5bc85c729aadad6b1e141fa949MD53ri/128052022-07-05 14:03:44.275oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestrepositorio@ufba.bropendoar:19322022-07-05T17:03:44Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Effects of lllt on the proliferation of hep2 cells: study in vitro
dc.title.alternative.pt_BR.fl_str_mv Proceedings of SPIE
title Effects of lllt on the proliferation of hep2 cells: study in vitro
spellingShingle Effects of lllt on the proliferation of hep2 cells: study in vitro
Pinheiro, Antonio Luiz Barbosa
Cell proliferation
Hazards
Stimulatory effect
title_short Effects of lllt on the proliferation of hep2 cells: study in vitro
title_full Effects of lllt on the proliferation of hep2 cells: study in vitro
title_fullStr Effects of lllt on the proliferation of hep2 cells: study in vitro
title_full_unstemmed Effects of lllt on the proliferation of hep2 cells: study in vitro
title_sort Effects of lllt on the proliferation of hep2 cells: study in vitro
author Pinheiro, Antonio Luiz Barbosa
author_facet Pinheiro, Antonio Luiz Barbosa
Nascimento, Silene Carneiro do
Viera, Alessandro Leonardo de Barros
Rolim, AluIzio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
author_role author
author2 Nascimento, Silene Carneiro do
Viera, Alessandro Leonardo de Barros
Rolim, AluIzio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro, Antonio Luiz Barbosa
Nascimento, Silene Carneiro do
Viera, Alessandro Leonardo de Barros
Rolim, AluIzio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
Pinheiro, Antonio Luiz Barbosa
Nascimento, Silene Carneiro do
Viera, Alessandro Leonardo de Barros
Rolim, AluIzio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
dc.subject.por.fl_str_mv Cell proliferation
Hazards
Stimulatory effect
topic Cell proliferation
Hazards
Stimulatory effect
description p. 75-81
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2013-09-03T12:01:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/12805
dc.identifier.number.pt_BR.fl_str_mv v. 3910
url http://www.repositorio.ufba.br/ri/handle/ri/12805
identifier_str_mv v. 3910
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Proceedings of SPIE
publisher.none.fl_str_mv Proceedings of SPIE
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/12805/1/88888888888888888888.pdf
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