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Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno

Bibliographic Details
Main Author: Santos, Vanderlei Saboia dos
Publication Date: 2017
Format: Master thesis
Language: por
Source: Repositório Institucional da Universidade do Estado do Amazonas (UEA)
Download full: https://ri.uea.edu.br/handle/riuea/2073
Summary: Basidiomycetes fungi are well known for their macroscopic forms, such as mushrooms and wood ear, and play an important role in the cycling of nutrients in ecosystems. Currently, studies report the importance of basidiomycetes in degrading environmental contaminants through the use set of low specificity ligninolytic enzymes. The objective of this work is to select basidiomycetes isolated from the Amazon region that present potential for Benzo(a)Pyrene (BaP) degradation. The isolates were pre-selected for the ability in oxidize gallic acid to quinone, this assay indicates the presence of ligninolytic enzymes important for degradation of xenobiotics, in sequence, the isolates were evaluated for potential to discolor the Remazol Brilliant Blue R (RBBR) dye derived from Anthracene. The selected fungi were used in assays to evaluate the activity of lignolytic enzymes (Lacase, Lignin Peroxidases and Manganese Peroxidase) in the presence of RBBR dye and BaP. Fungi that showed enzymatic activity in the presence of xenobiotics were selected for in vitro biodegradation assays of BaP, BaP metabolism analysis was also performed. Of the fungal isolates, 42% presented results for gallic acid oxidation with highlight for two fungal isolates (UEAF26-2 and UEAF06-2). The isolate UEAF26-2 showed production of MnP (12.73 IU L-1) and LiP (21.65 IU L-1) with highlight Lacase (78.4 IU L-1) in enzymatic activity assay with RBBR obtained 94% discoloration in 24 hours for the concentration of 100 mgL-1 of RBBR. In enzymatic activity assays using BaP as inducer the isolate presented LiP production (1.15 IU L-1), with emphasis on Lacase (28, 52 IU L-1). The isolate UEAF06-2 showed production for Lacase (22.17 IU L- 1), MnP (9.91 IU L-1) and LiP (8.89 IU L-1) and in 24 hours discolored 38% of the dye to 100 mgL-1 concentration of RBBR. In assays using BaP as an inducer of enzyme activity, the isolate obtained results for Lacase (4.46 IU L-1) and LiP (1.59 IU L-1). In a BaP degradation assay (1mg L-1), the isolate UEAF26-2 showed 9.9% in 7 days and 7.4% in 14 days. Isolate UEAF06-2 presented degradation of 13% in 7 days and 42.3% for 14 days with BaP (1mg L- 1). In analysis of BaP metabolites, the analyzed metabolites (coumarin and 1-hydroxysilane-2- naphthoic acid) were not detected. The results highlight the potential of isolate UEAF06-2 for degradation of BaP and the use of this fungus in later tests of degradation of other HPAs. Keywords: Basidiomycetes from Amazon, Ligninolytic enzymes, biodegradation, Benzo(a)Pyrene.
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spelling Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pirenoBasidiomicetosEnzimas ligninolíticasbiodegradaçãoBenzo(a)PirenobiotecnologiaBasidiomycetes fungi are well known for their macroscopic forms, such as mushrooms and wood ear, and play an important role in the cycling of nutrients in ecosystems. Currently, studies report the importance of basidiomycetes in degrading environmental contaminants through the use set of low specificity ligninolytic enzymes. The objective of this work is to select basidiomycetes isolated from the Amazon region that present potential for Benzo(a)Pyrene (BaP) degradation. The isolates were pre-selected for the ability in oxidize gallic acid to quinone, this assay indicates the presence of ligninolytic enzymes important for degradation of xenobiotics, in sequence, the isolates were evaluated for potential to discolor the Remazol Brilliant Blue R (RBBR) dye derived from Anthracene. The selected fungi were used in assays to evaluate the activity of lignolytic enzymes (Lacase, Lignin Peroxidases and Manganese Peroxidase) in the presence of RBBR dye and BaP. Fungi that showed enzymatic activity in the presence of xenobiotics were selected for in vitro biodegradation assays of BaP, BaP metabolism analysis was also performed. Of the fungal isolates, 42% presented results for gallic acid oxidation with highlight for two fungal isolates (UEAF26-2 and UEAF06-2). The isolate UEAF26-2 showed production of MnP (12.73 IU L-1) and LiP (21.65 IU L-1) with highlight Lacase (78.4 IU L-1) in enzymatic activity assay with RBBR obtained 94% discoloration in 24 hours for the concentration of 100 mgL-1 of RBBR. In enzymatic activity assays using BaP as inducer the isolate presented LiP production (1.15 IU L-1), with emphasis on Lacase (28, 52 IU L-1). The isolate UEAF06-2 showed production for Lacase (22.17 IU L- 1), MnP (9.91 IU L-1) and LiP (8.89 IU L-1) and in 24 hours discolored 38% of the dye to 100 mgL-1 concentration of RBBR. In assays using BaP as an inducer of enzyme activity, the isolate obtained results for Lacase (4.46 IU L-1) and LiP (1.59 IU L-1). In a BaP degradation assay (1mg L-1), the isolate UEAF26-2 showed 9.9% in 7 days and 7.4% in 14 days. Isolate UEAF06-2 presented degradation of 13% in 7 days and 42.3% for 14 days with BaP (1mg L- 1). In analysis of BaP metabolites, the analyzed metabolites (coumarin and 1-hydroxysilane-2- naphthoic acid) were not detected. The results highlight the potential of isolate UEAF06-2 for degradation of BaP and the use of this fungus in later tests of degradation of other HPAs. Keywords: Basidiomycetes from Amazon, Ligninolytic enzymes, biodegradation, Benzo(a)Pyrene.Os fungos basidiomicetos são bastante conhecidos por suas formas macroscópicas, tais como os cogumelos e orelha-de-pau, e desempenham um importante papel na ciclagem de nutrientes nos ecossistemas. Atualmente estudos relatam a importância dos basidiomicetos em degradar contaminantes ambientais, através do uso de um conjunto de enzimas ligninolíticas de baixa especificidade. O objetivo deste trabalho é selecionar basidiomicetos isolados da região Amazônica que apresentem potencial para degradação do Benzo(a)Pireno (BaP). Os isolados foram pré-selecionados pela capacidade em oxidar ácido gálico a quinona, esse teste indica a presença de enzimas ligninolíticas importantes para degradação de xenobióticos. Tambem foram avaliados seu potencial em descolorir o corante Azul brilhante de Remazol R (RBBR) derivado do Antraceno. Os fungos selecionados foram utilizados em ensaios para avaliar a atividade das enzimas lignolíticas Lacase, Lignina Peroxidases (LiP) e Manganês Peroxidase (MnP) na presença de corante RBBR e BaP. Os fungos que apresentaram atividade enzimática na presença dos xenobióticos foram selecionados para ensaios de biodegradação de BaP in vitro, a análise qualitativa de metabólitos de BaP também foi realizada. Dos isolados fúngicos, 42% apresentaram resultado para oxidação de ácido gálico com destaque para dois isolados fúngicos (UEAF26-2 e UEAF06-2). O isolado UEAF26-2 apresentou produção de MnP (12,73 UI L-1) e LiP (21,65 UI L-1) com destaque para Lacase (78,4 UI L-1) em ensaio de atividade enzimática com corante RBBR e 94% de descoloração em 24 horas para concentração de 100 mgL-1 de RBBR. O mesmo obteve produção das enzimas LiP (1,15 UI L-1), com destaque para Lacase (28,52 UI L-1), utilizando BaP como indutor. O isolado UEAF06-2 produziu Lacase (22,17 UI L-1), MnP (9,91 UI L-1) e LiP (8,89 UI L-1) com indutor RBBR e em 24 horas descoloriu 38% do corante para concentração 100 mgL-1 de RBBR. Em ensaios ulitizando BaP como indutor da atividade enzimática o isolado obteve resultado para Lacase (4,46 UI L-1) e LiP (1,59 UI L-1). Em ensaio de degradação de BaP (1mg L-1) o isolado UEAF26-2 apresentou 9,9% em 7 dias e 7,4% para 14 dias. O isolado UEAF06- 2 apresentou degradação de 13% em 7 dias e 42,3% para 14 dias com BaP (1mg L-1). Em análise de metabólitos do BaP, não foram detectados os metabólitos analisados (cumarina e ácido 1-hrodixi-2-naftóico). Os resultados destacam o potencial do isolado UEAF06-2 para degradação do BaP e a utilização desse fungo em ensaios posterios de degradação de outros HPAs. Palavras-chave: Basidiomicetos da Amazônia, Enzimas ligninolíticas, biodegradação, Benzo(a)Pireno.Universidade do Estado do AmazonasBrasilUEAPrograma de Pós-Graduação em Biotecnologia e Recursos naturais da AmazôniaZanotto, Sandra PatríciaBarroso, Hileia dos SantosZanotto, Sandra PatríciaVeiga Junior, Valdir FlorêncioProcópio, Rudi Emerson de LimaSantos, Vanderlei Saboia dos2020-03-19T00:41:21Z2024-09-05T17:29:20Z2020-03-182020-03-19T00:41:21Z2017-04-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://ri.uea.edu.br/handle/riuea/2073porACEVEDO, F., Pizzul, L., Castillo, M. del P., Cuevas, R. & Diez, M.C. Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor. Journal of Hazardous Materials 185: 212–219. 2011. ACEMIOGLU, B. Adsorption of Congo red from aqueous solution onto calcium-rich fly ash, J. Colloid Interface Sci. V.274, p.371-379, 2004. ANDRADE, J.A.; FABIO A.; JARDIM, S. F. 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dc.title.none.fl_str_mv Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
title Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
spellingShingle Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
Santos, Vanderlei Saboia dos
Basidiomicetos
Enzimas ligninolíticas
biodegradação
Benzo(a)Pireno
biotecnologia
title_short Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
title_full Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
title_fullStr Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
title_full_unstemmed Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
title_sort Seleção de basidiomicetos da região Amazônica com potencial para degradação de benzo(a)pireno
author Santos, Vanderlei Saboia dos
author_facet Santos, Vanderlei Saboia dos
author_role author
dc.contributor.none.fl_str_mv Zanotto, Sandra Patrícia
Barroso, Hileia dos Santos
Zanotto, Sandra Patrícia
Veiga Junior, Valdir Florêncio
Procópio, Rudi Emerson de Lima
dc.contributor.author.fl_str_mv Santos, Vanderlei Saboia dos
dc.subject.por.fl_str_mv Basidiomicetos
Enzimas ligninolíticas
biodegradação
Benzo(a)Pireno
biotecnologia
topic Basidiomicetos
Enzimas ligninolíticas
biodegradação
Benzo(a)Pireno
biotecnologia
description Basidiomycetes fungi are well known for their macroscopic forms, such as mushrooms and wood ear, and play an important role in the cycling of nutrients in ecosystems. Currently, studies report the importance of basidiomycetes in degrading environmental contaminants through the use set of low specificity ligninolytic enzymes. The objective of this work is to select basidiomycetes isolated from the Amazon region that present potential for Benzo(a)Pyrene (BaP) degradation. The isolates were pre-selected for the ability in oxidize gallic acid to quinone, this assay indicates the presence of ligninolytic enzymes important for degradation of xenobiotics, in sequence, the isolates were evaluated for potential to discolor the Remazol Brilliant Blue R (RBBR) dye derived from Anthracene. The selected fungi were used in assays to evaluate the activity of lignolytic enzymes (Lacase, Lignin Peroxidases and Manganese Peroxidase) in the presence of RBBR dye and BaP. Fungi that showed enzymatic activity in the presence of xenobiotics were selected for in vitro biodegradation assays of BaP, BaP metabolism analysis was also performed. Of the fungal isolates, 42% presented results for gallic acid oxidation with highlight for two fungal isolates (UEAF26-2 and UEAF06-2). The isolate UEAF26-2 showed production of MnP (12.73 IU L-1) and LiP (21.65 IU L-1) with highlight Lacase (78.4 IU L-1) in enzymatic activity assay with RBBR obtained 94% discoloration in 24 hours for the concentration of 100 mgL-1 of RBBR. In enzymatic activity assays using BaP as inducer the isolate presented LiP production (1.15 IU L-1), with emphasis on Lacase (28, 52 IU L-1). The isolate UEAF06-2 showed production for Lacase (22.17 IU L- 1), MnP (9.91 IU L-1) and LiP (8.89 IU L-1) and in 24 hours discolored 38% of the dye to 100 mgL-1 concentration of RBBR. In assays using BaP as an inducer of enzyme activity, the isolate obtained results for Lacase (4.46 IU L-1) and LiP (1.59 IU L-1). In a BaP degradation assay (1mg L-1), the isolate UEAF26-2 showed 9.9% in 7 days and 7.4% in 14 days. Isolate UEAF06-2 presented degradation of 13% in 7 days and 42.3% for 14 days with BaP (1mg L- 1). In analysis of BaP metabolites, the analyzed metabolites (coumarin and 1-hydroxysilane-2- naphthoic acid) were not detected. The results highlight the potential of isolate UEAF06-2 for degradation of BaP and the use of this fungus in later tests of degradation of other HPAs. Keywords: Basidiomycetes from Amazon, Ligninolytic enzymes, biodegradation, Benzo(a)Pyrene.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-28
2020-03-19T00:41:21Z
2020-03-18
2020-03-19T00:41:21Z
2024-09-05T17:29:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ri.uea.edu.br/handle/riuea/2073
url https://ri.uea.edu.br/handle/riuea/2073
dc.language.iso.fl_str_mv por
language por
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dc.rights.driver.fl_str_mv Atribuição-NãoComercial-SemDerivados 3.0 Brasil
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribuição-NãoComercial-SemDerivados 3.0 Brasil
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade do Estado do Amazonas
Brasil
UEA
Programa de Pós-Graduação em Biotecnologia e Recursos naturais da Amazônia
publisher.none.fl_str_mv Universidade do Estado do Amazonas
Brasil
UEA
Programa de Pós-Graduação em Biotecnologia e Recursos naturais da Amazônia
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade do Estado do Amazonas (UEA)
instname:Universidade do Estado do Amazonas (UEA)
instacron:UEA
instname_str Universidade do Estado do Amazonas (UEA)
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institution UEA
reponame_str Repositório Institucional da Universidade do Estado do Amazonas (UEA)
collection Repositório Institucional da Universidade do Estado do Amazonas (UEA)
repository.name.fl_str_mv Repositório Institucional da Universidade do Estado do Amazonas (UEA) - Universidade do Estado do Amazonas (UEA)
repository.mail.fl_str_mv bibliotecacentral@uea.edu.br
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