Influence of experimental infection by Haemonchus contortus on acetylcholinesterase activity in lymphocytes of lambs

• Main Author: Tonin A.A.
• Publication Date: 2014
• Other Authors: Da Silva A.S.*, Schafer A.S., Aires A.R., Oliveira C.B., Zanini D., Schetinger M.R.C., Morsch V.M., Lopes S.T.A., Monteiro S.G., Leal M.L.R.
• Format: Article
• Language: eng
• Source: Repositório Institucional da Udesc
• dARK ID: ark:/33523/001300000bs52
• Download full: https://repositorio.udesc.br/handle/UDESC/8426

The aim of this study was to evaluate the acetylcholinesterase (AChE) activity in lymphocytes of lambs experimentally infected by Haemonchus contortus. A total of 14 healthy lambs were used, divided into two groups of seven animals each. Group A (negative control) represented the uninfected animals, and Group B (positive control) was formed by animals infected with 15,000 larvae of H. contortus. Blood was drawn on the days 15, 45 and 75 post-infection (PI) in order to perform the white blood cells (WBC) count, as well as the evaluation of AChE activity in lymphocytes. Parasitological stool exam (eggs per gram of feces - EPG) was performed on the same days to follow up the evolution of the infection. On day 15 PI it was verified negative EPG; however, on days 45 and 75 PI it was observed positive EPG only in the animals of group B. In the three evaluated periods was observed a lower number of leukocytes, associated with decreased lymphocytes and neutrophils in lambs infected by this gastrointestinal nematodes. Lambs infected with H. contortus showed significant (P<. 0.01) lower AChE activity in lymphocytes compared uninfected. Statistically, there was a positive correlation (P<. 0.05) between AChE activity in lymphocytes and number of lymphocytes (r= 0.69). The lymphocytes are cells with direct participation in the cholinergic system; therefore, based on these results, it can be concluded that the experimental infection with H. contortus influences the number of lymphocytes, and consequently the AChE activity in these cells. © 2014 Elsevier Inc.