Iron metabolism and its relationship to anemia and immune system in Trypanosoma evansi infected rats

• Main Author: da Silva C.B.
• Publication Date: 2013
• Other Authors: Wolkmer P., Paim F.C., Da Silva A.S.*, Siqueira L.C., de Souza C.L., Franca R.T., Dornelles G.L., Medeiros Frescura Duarte M.M., Monteiro S.G., Mazzanti C.M., dos Anjos Lopes S.T.
• Format: Article
• Language: eng
• Source: Repositório Institucional da Udesc
• Download full: https://repositorio.udesc.br/handle/UDESC/9079

The aim of this study was to evaluate biochemical parameters of iron metabolism in rats experimentally infected with Trypanosoma evansi. To this end, 20 rats (Wistar) were intraperitoneally inoculated with blood containing trypomastigotes 106 (Group T) and 12 animals were used as negative control (Group C) and received saline (0.2mL) through same route. Blood samples were collected by cardiac puncture on day 5 (C5, T5) and 30 (C30, T30) post-inoculation (pi) to perform complete blood count and determination of serum iron, transferrin, ferritin, total and latent iron fixation capacity, transferrin saturation and prohepcidin concentration. Also, bone marrow samples were collected, to perform Pearls staining reaction. Levels of iron, total and latent iron binding capacity and prohepcidin concentration were lower (P<0.05) in infected rats (T5 and T30 groups) compared to controls. On the other hand, levels of transferrin and ferritin were higher when compared to controls (P<0.05). The transferrin saturation increased on day 5pi, but decreased on day 30pi. The Pearls reaction showed a higher accumulation of iron in the bone marrow of infected animals in day 5pi (P<0.01). Infection with T. evansi in rats caused anemia and changes in iron metabolism associated to the peaks of parasitemia. These results suggest that changes in iron metabolism may be related to the host immune response to infection and anemic status of infected animals. © 2012 Elsevier Inc.