Comparison of different methodologies for DNA extraction from Aegla longirostri

Bibliographic Details
Main Author: Bitencourt,João Vitor Trindade
Publication Date: 2007
Other Authors: Roratto,Paula Angélica, Bartholomei-Santos,Marlise Ladvocat, Santos,Sandro
Format: Article
Language: eng
Source: Brazilian Archives of Biology and Technology
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010
Summary: The aim of this study was to compare some DNA extraction methodologies for Aegla longirostri. The protocols were based on the traditional phenol-chloroform DNA extraction methodology and using a commercial kit for DNA extraction. They differed in tissues used, the addition - or not - of beta-mercaptoethanol to the lysis buffer, times and methods for the animal's conservation (frozen, in ethanol or fresh). Individuals stored at -20°C for a long time supplied lower molecular weight DNA than those stored for a short time. The best yield for the specimens preserved in ethanol was obtained for 15 days storage in 95% ethanol. The kit resulted in a low quantity of high molecular weight DNA. The best protocol for DNA extraction from Aeglidae, and probably for other crustaceans should, therefore, utilize fresh specimens, with addition of beta-mercaptoethanol to the lysis buffer.
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spelling Comparison of different methodologies for DNA extraction from Aegla longirostriAnomuraAeglidaefreshwater crabDNA extractionThe aim of this study was to compare some DNA extraction methodologies for Aegla longirostri. The protocols were based on the traditional phenol-chloroform DNA extraction methodology and using a commercial kit for DNA extraction. They differed in tissues used, the addition - or not - of beta-mercaptoethanol to the lysis buffer, times and methods for the animal's conservation (frozen, in ethanol or fresh). Individuals stored at -20°C for a long time supplied lower molecular weight DNA than those stored for a short time. The best yield for the specimens preserved in ethanol was obtained for 15 days storage in 95% ethanol. The kit resulted in a low quantity of high molecular weight DNA. The best protocol for DNA extraction from Aeglidae, and probably for other crustaceans should, therefore, utilize fresh specimens, with addition of beta-mercaptoethanol to the lysis buffer.Instituto de Tecnologia do Paraná - Tecpar2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010Brazilian Archives of Biology and Technology v.50 n.6 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000700010info:eu-repo/semantics/openAccessBitencourt,João Vitor TrindadeRoratto,Paula AngélicaBartholomei-Santos,Marlise LadvocatSantos,Sandroeng2008-03-03T00:00:00Zoai:scielo:S1516-89132007000700010Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-03-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Comparison of different methodologies for DNA extraction from Aegla longirostri
title Comparison of different methodologies for DNA extraction from Aegla longirostri
spellingShingle Comparison of different methodologies for DNA extraction from Aegla longirostri
Bitencourt,João Vitor Trindade
Anomura
Aeglidae
freshwater crab
DNA extraction
title_short Comparison of different methodologies for DNA extraction from Aegla longirostri
title_full Comparison of different methodologies for DNA extraction from Aegla longirostri
title_fullStr Comparison of different methodologies for DNA extraction from Aegla longirostri
title_full_unstemmed Comparison of different methodologies for DNA extraction from Aegla longirostri
title_sort Comparison of different methodologies for DNA extraction from Aegla longirostri
author Bitencourt,João Vitor Trindade
author_facet Bitencourt,João Vitor Trindade
Roratto,Paula Angélica
Bartholomei-Santos,Marlise Ladvocat
Santos,Sandro
author_role author
author2 Roratto,Paula Angélica
Bartholomei-Santos,Marlise Ladvocat
Santos,Sandro
author2_role author
author
author
dc.contributor.author.fl_str_mv Bitencourt,João Vitor Trindade
Roratto,Paula Angélica
Bartholomei-Santos,Marlise Ladvocat
Santos,Sandro
dc.subject.por.fl_str_mv Anomura
Aeglidae
freshwater crab
DNA extraction
topic Anomura
Aeglidae
freshwater crab
DNA extraction
description The aim of this study was to compare some DNA extraction methodologies for Aegla longirostri. The protocols were based on the traditional phenol-chloroform DNA extraction methodology and using a commercial kit for DNA extraction. They differed in tissues used, the addition - or not - of beta-mercaptoethanol to the lysis buffer, times and methods for the animal's conservation (frozen, in ethanol or fresh). Individuals stored at -20°C for a long time supplied lower molecular weight DNA than those stored for a short time. The best yield for the specimens preserved in ethanol was obtained for 15 days storage in 95% ethanol. The kit resulted in a low quantity of high molecular weight DNA. The best protocol for DNA extraction from Aeglidae, and probably for other crustaceans should, therefore, utilize fresh specimens, with addition of beta-mercaptoethanol to the lysis buffer.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000700010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.6 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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