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Culture medium for amylase production by toxigenic fungi

Detalhes bibliográficos
Autor(a) principal: Figueira,Edson Luiz Zangrando
Data de Publicação: 2000
Outros Autores: Hirooka,Elisa Yoko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003
Resumo: Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day of fermentation, both for F. moniliforme (42.32U/mL) and A. flavus (4,745.54U/mL).
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spelling Culture medium for amylase production by toxigenic fungiAmylaseFusarium moniliformeAspergillus flavusMycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day of fermentation, both for F. moniliforme (42.32U/mL) and A. flavus (4,745.54U/mL).Instituto de Tecnologia do Paraná - Tecpar2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003Brazilian Archives of Biology and Technology v.43 n.5 2000reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132000000500003info:eu-repo/semantics/openAccessFigueira,Edson Luiz ZangrandoHirooka,Elisa Yokoeng2001-10-25T00:00:00Zoai:scielo:S1516-89132000000500003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2001-10-25T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Culture medium for amylase production by toxigenic fungi
title Culture medium for amylase production by toxigenic fungi
spellingShingle Culture medium for amylase production by toxigenic fungi
Figueira,Edson Luiz Zangrando
Amylase
Fusarium moniliforme
Aspergillus flavus
title_short Culture medium for amylase production by toxigenic fungi
title_full Culture medium for amylase production by toxigenic fungi
title_fullStr Culture medium for amylase production by toxigenic fungi
title_full_unstemmed Culture medium for amylase production by toxigenic fungi
title_sort Culture medium for amylase production by toxigenic fungi
author Figueira,Edson Luiz Zangrando
author_facet Figueira,Edson Luiz Zangrando
Hirooka,Elisa Yoko
author_role author
author2 Hirooka,Elisa Yoko
author2_role author
dc.contributor.author.fl_str_mv Figueira,Edson Luiz Zangrando
Hirooka,Elisa Yoko
dc.subject.por.fl_str_mv Amylase
Fusarium moniliforme
Aspergillus flavus
topic Amylase
Fusarium moniliforme
Aspergillus flavus
description Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day of fermentation, both for F. moniliforme (42.32U/mL) and A. flavus (4,745.54U/mL).
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132000000500003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.43 n.5 2000
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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