Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19

Bibliographic Details
Main Author: Campos, Gubio
Publication Date: 2020
Other Authors: Sardi, Silvia, Falcao, Melissa, Belitardo, Emilia, Rocha, Danilo, Rolo, Carolina, Menezes, Aline, Pinheiro, Carina, Carvalho, Rejane, Almeida, João, Aguiar, Eric, Pacheco, Luis
Format: preprint
Language: eng
Source: SciELO Preprints
Download full: https://preprints.scielo.org/index.php/scielo/preprint/view/239
Summary: Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
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spelling Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19Covid-19SARS-CoV-2next-generation sequencingmetatranscriptomicsHerein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.SciELO PreprintsSciELO PreprintsSciELO Preprints2020-04-29info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/23910.1590/SciELOPreprints.239enghttps://preprints.scielo.org/index.php/scielo/article/view/239/291Copyright (c) 2020 Gubio S. Campos, Silvia I. Sardi, Melissa B. Falcao, Emilia M. M. A. Belitardo, Danilo J. P. G. Rocha, Carolina A. Rolo, Aline D. Menezes, Carina Pinheiro, Rejane H. Carvalho, João P. P. Almeida, Eric R. G. R. Aguiar, Luis G. C. Pachecohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCampos, GubioSardi, SilviaFalcao, MelissaBelitardo, EmiliaRocha, DaniloRolo, CarolinaMenezes, AlinePinheiro, CarinaCarvalho, RejaneAlmeida, JoãoAguiar, EricPacheco, Luisreponame:SciELO Preprintsinstname:SciELOinstacron:SCI2020-04-28T18:04:52Zoai:ops.preprints.scielo.org:preprint/239Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2020-04-28T18:04:52SciELO Preprints - SciELOfalse
dc.title.none.fl_str_mv Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
title Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
spellingShingle Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
Campos, Gubio
Covid-19
SARS-CoV-2
next-generation sequencing
metatranscriptomics
title_short Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
title_full Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
title_fullStr Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
title_full_unstemmed Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
title_sort Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
author Campos, Gubio
author_facet Campos, Gubio
Sardi, Silvia
Falcao, Melissa
Belitardo, Emilia
Rocha, Danilo
Rolo, Carolina
Menezes, Aline
Pinheiro, Carina
Carvalho, Rejane
Almeida, João
Aguiar, Eric
Pacheco, Luis
author_role author
author2 Sardi, Silvia
Falcao, Melissa
Belitardo, Emilia
Rocha, Danilo
Rolo, Carolina
Menezes, Aline
Pinheiro, Carina
Carvalho, Rejane
Almeida, João
Aguiar, Eric
Pacheco, Luis
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campos, Gubio
Sardi, Silvia
Falcao, Melissa
Belitardo, Emilia
Rocha, Danilo
Rolo, Carolina
Menezes, Aline
Pinheiro, Carina
Carvalho, Rejane
Almeida, João
Aguiar, Eric
Pacheco, Luis
dc.subject.por.fl_str_mv Covid-19
SARS-CoV-2
next-generation sequencing
metatranscriptomics
topic Covid-19
SARS-CoV-2
next-generation sequencing
metatranscriptomics
description Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/preprint
info:eu-repo/semantics/publishedVersion
format preprint
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://preprints.scielo.org/index.php/scielo/preprint/view/239
10.1590/SciELOPreprints.239
url https://preprints.scielo.org/index.php/scielo/preprint/view/239
identifier_str_mv 10.1590/SciELOPreprints.239
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://preprints.scielo.org/index.php/scielo/article/view/239/291
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
dc.source.none.fl_str_mv reponame:SciELO Preprints
instname:SciELO
instacron:SCI
instname_str SciELO
instacron_str SCI
institution SCI
reponame_str SciELO Preprints
collection SciELO Preprints
repository.name.fl_str_mv SciELO Preprints - SciELO
repository.mail.fl_str_mv scielo.submission@scielo.org
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