Ochratoxin A determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography

• Main Author: Prado,Guilherme
• Publication Date: 2003
• Other Authors: Oliveira,Marize Silva, Carvalho,Eliana Pinheiro, Lima,Luiz Carlos Oliveira, Veloso,Thais, Souza,Leandro Augusto Ferreira, Cardoso,Ana Cristina Ferreira
• Format: Article
• Language: eng
• Source: Food Science and Technology (Campinas)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000400011
• topic: ochratoxin A; beer; immunoaffinity; HPLC

Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficient of variation between 0% e 11.0%. Detection limit and quantification limit were 2.0pg/mL and 8.0pg/mL, respectively. Of the total of 26 samples produced in Brazil only 6 (23%), contained trace amounts of OTA. Of the 4 imported beers, in 2, Ireland and Germany, were detected OTA at levels of 25pg/mL and 82pg/mL, respectively.