Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.

Bibliographic Details
Main Author: Sousa, M. F.
Publication Date: 2004
Other Authors: Silva, Paulo, Façanha, Arnoldo, Tavares, R. M., Neto, T. Lino, Gerós, H.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/2229
Summary: Poster apresentado no congresso "14th Congress of the Federation of European Societies of Plant Biology", August 23-27, 2004, Cracow, Poland.
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spelling Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.Phosphate transportHakea sericeaProteoid rootsScience & TechnologyPoster apresentado no congresso "14th Congress of the Federation of European Societies of Plant Biology", August 23-27, 2004, Cracow, Poland.P is an important macronutrient, making up about 0.2% of a plant’s dry weight. The form of P most readily accessed by plants is Pi, the concentration of which rarely exceeds 10 µM in soil (Schachtman et al., 1998). Here we studied the mechanisms involved on extraction of Pi from soil in Hakea sericea Schrad. (Proteacea), an Australian invader of natural habitats, which is able to develop proteoid roots as a response to P deficiency. Material and Methods: Proteoid roots were harvested from adult H. sericea shrubs growing in Serra d’Arga, Northern Portugal. The P uptake buffer contained 2.5-4000 µM NaH2PO4 and Pi concentration was determined according to Adams (1991). PCR amplification was performed using genomic DNA and degenerate primers based on the most conserved regions of plant P transporters genes. Results and Conclusions: H. sericea proteoid roots have highly efficient transporters for extraction of Pi from soil. Kinetic studies supported the involvement of two Pi mediated transport systems associated with a diffusion-like mechanism. The kinetic parameters were as follows: for the high-affinity system Km, 11 µM Pi and Vmax, 7 µmol h-1 g-1 FW; for the low-affinity system Km, 163 µM Pi and Vmax, 30 µmol h-1 g-1 FW. The protonophore CCCP (50 µM) inhibited up to 60% the initial uptake rates of 5-50 µM Pi (high-affinity range) and 100-500 µM (low-affinity range), suggesting H+-dependent transport. Both transporters were competitively inhibited by phosphite and 100 µM mersalyl reduced by 50% the initial uptake rates of 10 µM Pi. PCR using degenerate primers allowed the amplification of two 438 bp fragments sharing 87.4% identity with each other. Both nucleotide sequences comprise an incomplete ORF coding for transmembrane domains and exhibiting 85-82% identity with other plant P transporters. These nucleotide sequences are being used for the identification of complete cDNAs of Pi transporter genes of H. sericea.Fundação para a Ciência e a Tecnologia (FCT) - Bolsa Ref. SFRH/BD/10899/2002 , Bolsa Ref. SFRH/BD/13460/2003.Universidade do MinhoSousa, M. F.Silva, PauloFaçanha, ArnoldoTavares, R. M.Neto, T. LinoGerós, H.2004-08-232004-08-23T00:00:00Zconference posterinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/2229enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:27:11Zoai:repositorium.sdum.uminho.pt:1822/2229Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:18:47.683598Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
title Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
spellingShingle Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
Sousa, M. F.
Phosphate transport
Hakea sericea
Proteoid roots
Science & Technology
title_short Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
title_full Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
title_fullStr Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
title_full_unstemmed Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
title_sort Orthophosphate uptake in proteoid roots of naturally occurring Hakea sericea Schrad.
author Sousa, M. F.
author_facet Sousa, M. F.
Silva, Paulo
Façanha, Arnoldo
Tavares, R. M.
Neto, T. Lino
Gerós, H.
author_role author
author2 Silva, Paulo
Façanha, Arnoldo
Tavares, R. M.
Neto, T. Lino
Gerós, H.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sousa, M. F.
Silva, Paulo
Façanha, Arnoldo
Tavares, R. M.
Neto, T. Lino
Gerós, H.
dc.subject.por.fl_str_mv Phosphate transport
Hakea sericea
Proteoid roots
Science & Technology
topic Phosphate transport
Hakea sericea
Proteoid roots
Science & Technology
description Poster apresentado no congresso "14th Congress of the Federation of European Societies of Plant Biology", August 23-27, 2004, Cracow, Poland.
publishDate 2004
dc.date.none.fl_str_mv 2004-08-23
2004-08-23T00:00:00Z
dc.type.driver.fl_str_mv conference poster
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2229
url http://hdl.handle.net/1822/2229
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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repository.mail.fl_str_mv info@rcaap.pt
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