Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR

Bibliographic Details
Main Author: Jorge, Lurdes
Publication Date: 2010
Other Authors: Gonzalez, Francisco J., Monte, Enrique, Choupina, Altino
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10198/3676
Summary: Trichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soilborne plant pathogens. Its biological control against plant fungi pathogens is based on different mechanisms, namely the production of several lytic enzymes. In T. harzianum several glucanases, celulases, chitinases and proteases has been identified but little is known about its lipolytic system. The aim of this work was to achieve the complete elucidation of T. harzianum lip2 by HE-TAIL PCR (High-Efficiency Thermal Asymetric Interlaced PCR), a method described as efficient to identify flanking regions from short known DNA sequences
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spelling Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCRTrichoderma harzianumHE-TAIL PCRlip2Trichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soilborne plant pathogens. Its biological control against plant fungi pathogens is based on different mechanisms, namely the production of several lytic enzymes. In T. harzianum several glucanases, celulases, chitinases and proteases has been identified but little is known about its lipolytic system. The aim of this work was to achieve the complete elucidation of T. harzianum lip2 by HE-TAIL PCR (High-Efficiency Thermal Asymetric Interlaced PCR), a method described as efficient to identify flanking regions from short known DNA sequencesBiblioteca Digital do IPBJorge, LurdesGonzalez, Francisco J.Monte, EnriqueChoupina, Altino2011-03-21T10:30:14Z20102010-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10198/3676engJorge, Lurdes; González, Francisco; Monte, Enrique; Choupina, Altino (2010). Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR. In XVII National Congress of Biochemistry. Portoinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-25T11:56:30Zoai:bibliotecadigital.ipb.pt:10198/3676Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T11:18:34.737259Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
title Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
spellingShingle Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
Jorge, Lurdes
Trichoderma harzianum
HE-TAIL PCR
lip2
title_short Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
title_full Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
title_fullStr Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
title_full_unstemmed Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
title_sort Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
author Jorge, Lurdes
author_facet Jorge, Lurdes
Gonzalez, Francisco J.
Monte, Enrique
Choupina, Altino
author_role author
author2 Gonzalez, Francisco J.
Monte, Enrique
Choupina, Altino
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Jorge, Lurdes
Gonzalez, Francisco J.
Monte, Enrique
Choupina, Altino
dc.subject.por.fl_str_mv Trichoderma harzianum
HE-TAIL PCR
lip2
topic Trichoderma harzianum
HE-TAIL PCR
lip2
description Trichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soilborne plant pathogens. Its biological control against plant fungi pathogens is based on different mechanisms, namely the production of several lytic enzymes. In T. harzianum several glucanases, celulases, chitinases and proteases has been identified but little is known about its lipolytic system. The aim of this work was to achieve the complete elucidation of T. harzianum lip2 by HE-TAIL PCR (High-Efficiency Thermal Asymetric Interlaced PCR), a method described as efficient to identify flanking regions from short known DNA sequences
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2011-03-21T10:30:14Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/3676
url http://hdl.handle.net/10198/3676
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Jorge, Lurdes; González, Francisco; Monte, Enrique; Choupina, Altino (2010). Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR. In XVII National Congress of Biochemistry. Porto
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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