HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene

Bibliographic Details
Main Author: Jorge, Lurdes
Publication Date: 2008
Other Authors: Gonzalez, Francisco J., Monte, Enrique, Choupina, Altino
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10198/3686
Summary: Selected comunication. Oral presentation.
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spelling HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 geneTrichoderma harzianumLip1Biocontrol agentHE-TAIL PCRSelected comunication. Oral presentation.Trichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soilborne plant pathogens. Species of Trichoderma are commercially applied as biological control agents against plant fungal pathogens based on different mechanisms, such as antibiosis, competition for nutrients and mycoparasitism. One of the mechanisms involved is the production of several lytic enzymes. In T. harzianum has been identified several glucanases, celulases, chitinases and proteases, but nothing are already known about its lipolytic system. In this work we described the identification of lip1 from T. harzianum, a gene encoding the first extracellular triacylglicerol lipase known in this specie. A cDNA library of T. harzianum CECT 2413 cloned in pBluescript SK+ was screened, and the expressed sequence tags (ESTs) with more similarity against lipase gene sequences in international databases selected and sequenced. Elucidation of complete gene nucleotide sequence of lip1, including 52 bp of the open reading frame at the N-terminal region and 693 bp of the promoter region were achieved by high-efficiency thermal asymmetric interlaced (HE-TAIL) PCR (Michiels et al., 2003), which confirmed to be a powerful tool to identify flanking regions from short known sequences. Lip1 codifies a 558 amino acids protein, with 59322,6 KDa and a calculated global iso-electric point value of 4,56. The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AM18087.COMBATINTA/SP2.P11/02 Interreg IIIA (PC007) - 220201Biblioteca Digital do IPBJorge, LurdesGonzalez, Francisco J.Monte, EnriqueChoupina, Altino2011-03-21T11:48:29Z20082008-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10198/3686engJorge, Lurdes; González, Francisco; Monte, Enrique; Choupina, Altino (2008). HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene. In XVI Congresso Nacional de Bioquímica. Ponta Delgadainfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-25T11:56:32Zoai:bibliotecadigital.ipb.pt:10198/3686Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T11:18:37.816591Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
title HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
spellingShingle HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
Jorge, Lurdes
Trichoderma harzianum
Lip1
Biocontrol agent
HE-TAIL PCR
title_short HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
title_full HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
title_fullStr HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
title_full_unstemmed HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
title_sort HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene
author Jorge, Lurdes
author_facet Jorge, Lurdes
Gonzalez, Francisco J.
Monte, Enrique
Choupina, Altino
author_role author
author2 Gonzalez, Francisco J.
Monte, Enrique
Choupina, Altino
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Jorge, Lurdes
Gonzalez, Francisco J.
Monte, Enrique
Choupina, Altino
dc.subject.por.fl_str_mv Trichoderma harzianum
Lip1
Biocontrol agent
HE-TAIL PCR
topic Trichoderma harzianum
Lip1
Biocontrol agent
HE-TAIL PCR
description Selected comunication. Oral presentation.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2011-03-21T11:48:29Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/3686
url http://hdl.handle.net/10198/3686
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Jorge, Lurdes; González, Francisco; Monte, Enrique; Choupina, Altino (2008). HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 gene. In XVI Congresso Nacional de Bioquímica. Ponta Delgada
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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