Does impedance matter when recording spikes with polytrodes?

Bibliographic Details
Main Author: Neto, Joana P.
Publication Date: 2018
Other Authors: Baião, Pedro, Lopes, Gonçalo, Frazão, João, Nogueira, Joana, Fortunato, Elvira, Barquinha, Pedro, Kampff, Adam R.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://doi.org/10.3389/fnins.2018.00715
Summary: This work was supported by funding from the European Union's Seventh Framework Programme (FP7/2007-2013) Grant Agreement 600925, the Bial Foundation Grant 190/12 and the FCT-MCTES Doctoral Grant SFRH/BD/76004/2011 (to JN).
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spelling Does impedance matter when recording spikes with polytrodes?CoatingImpedanceMicroelectrodesNoiseSpikesNeuroscience(all)This work was supported by funding from the European Union's Seventh Framework Programme (FP7/2007-2013) Grant Agreement 600925, the Bial Foundation Grant 190/12 and the FCT-MCTES Doctoral Grant SFRH/BD/76004/2011 (to JN).Extracellular microelectrodes have been widely used to measure brain activity, yet there are still basic questions about the requirements for a good extracellular microelectrode. One common source of confusion is how much an electrode's impedance affects the amplitude of extracellular spikes and background noise. Here we quantify the effect of an electrode's impedance on data quality in extracellular recordings, which is crucial for both the detection of spikes and their assignment to the correct neurons. This study employs commercial polytrodes containing 32 electrodes (177 μm2) arranged in a dense array. This allowed us to directly compare, side-by-side, the same extracellular signals measured by modified low impedance (∼100 kΩ) microelectrodes with unmodified high impedance (∼1 MΩ) microelectrodes. We begin with an evaluation of existing protocols to lower the impedance of the electrodes. The poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT-PSS) electrodeposition protocol is a simple, stable, and reliable method for decreasing the impedance of a microelectrode up to 10-fold. We next record in vivo using polytrodes that are modified in a 'chess board' pattern, such that the signal of one neuron is detected by multiple coated and non-coated electrodes. The performance of the coated and non-coated electrodes is then compared on measures of background noise and amplitude of the detected action potentials. If the proper recording system is used, then the impedance of a microelectrode within the range of standard polytrodes (∼0.1 to 2 MΩ) does not greatly affect data quality and spike sorting. This study should encourage neuroscientists to stop worrying about one more unknown.UNINOVA-Instituto de Desenvolvimento de Novas TecnologiasCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisRUNNeto, Joana P.Baião, PedroLopes, GonçaloFrazão, JoãoNogueira, JoanaFortunato, ElviraBarquinha, PedroKampff, Adam R.2019-02-11T23:29:41Z2018-10-082018-10-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3389/fnins.2018.00715eng1662-4548PURE: 11471374http://www.scopus.com/inward/record.url?scp=85055145075&partnerID=8YFLogxKhttps://doi.org/10.3389/fnins.2018.00715info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:37:10Zoai:run.unl.pt:10362/60290Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:08:11.714765Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Does impedance matter when recording spikes with polytrodes?
title Does impedance matter when recording spikes with polytrodes?
spellingShingle Does impedance matter when recording spikes with polytrodes?
Neto, Joana P.
Coating
Impedance
Microelectrodes
Noise
Spikes
Neuroscience(all)
title_short Does impedance matter when recording spikes with polytrodes?
title_full Does impedance matter when recording spikes with polytrodes?
title_fullStr Does impedance matter when recording spikes with polytrodes?
title_full_unstemmed Does impedance matter when recording spikes with polytrodes?
title_sort Does impedance matter when recording spikes with polytrodes?
author Neto, Joana P.
author_facet Neto, Joana P.
Baião, Pedro
Lopes, Gonçalo
Frazão, João
Nogueira, Joana
Fortunato, Elvira
Barquinha, Pedro
Kampff, Adam R.
author_role author
author2 Baião, Pedro
Lopes, Gonçalo
Frazão, João
Nogueira, Joana
Fortunato, Elvira
Barquinha, Pedro
Kampff, Adam R.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
RUN
dc.contributor.author.fl_str_mv Neto, Joana P.
Baião, Pedro
Lopes, Gonçalo
Frazão, João
Nogueira, Joana
Fortunato, Elvira
Barquinha, Pedro
Kampff, Adam R.
dc.subject.por.fl_str_mv Coating
Impedance
Microelectrodes
Noise
Spikes
Neuroscience(all)
topic Coating
Impedance
Microelectrodes
Noise
Spikes
Neuroscience(all)
description This work was supported by funding from the European Union's Seventh Framework Programme (FP7/2007-2013) Grant Agreement 600925, the Bial Foundation Grant 190/12 and the FCT-MCTES Doctoral Grant SFRH/BD/76004/2011 (to JN).
publishDate 2018
dc.date.none.fl_str_mv 2018-10-08
2018-10-08T00:00:00Z
2019-02-11T23:29:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.3389/fnins.2018.00715
url https://doi.org/10.3389/fnins.2018.00715
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1662-4548
PURE: 11471374
http://www.scopus.com/inward/record.url?scp=85055145075&partnerID=8YFLogxK
https://doi.org/10.3389/fnins.2018.00715
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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