3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering

Detalhes bibliográficos
Autor(a) principal: Serafin, Aleksandra
Data de Publicação: 2023
Outros Autores: Culebras, Mario, Oliveira, Joaquim M., Koffler, Jacob, Collins, Maurice N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: https://hdl.handle.net/1822/85734
Resumo: Electrically conductive bio-scaffolds are explored in the field of tissue engineering (TE) as a solution to address the clinical need of electroactive tissues, finding applications in nervous, cardiac, and spinal cord injury repair. In this work, we synthesise polypyrrole nanoparticles (PPy NP) via the mini-emulsion method with further combination with a gelatin/hyaluronic acid (HA) hydrogel to create electroconductive Gel:HA:PPy-NP TE scaffolds. Electroconductive Gel:HA:PPy-NP scaffolds possess excellent mechanical properties at 1.08 ± 0.26 MPa, closely matching the reported mechanical performance of the spinal cord. Scaffolds were designed with controlled porosity of 526.2 ± 74.6â 403.9 ± 57.4 μm, and conductivities of 4.3 à 10 â 6 ± 1.1 à 10 â 6 S.cm â 1 were reached. Rheological studies show that prior to lyophilisation, the Gel:HA:PPy-NP hydrogels display a shear-thinning behaviour. These gels were subsequently 3D printed into predefined 2 layer lattice geometries and displayed excellent post-printing shape fidelity. In vitro studies show that the Gel:HA:PPy-NP scaffolds are cytocompatible with mesenchymal stem cells and neuronal stem cells and display encouraging cell attachment and proliferation profiles. Based on these results, the incorporation of PPy NPs into Gel:HA biomaterial scaffolds enhances the conductive capabilities of the material, while showcasing biocompatible behaviour with cell cultures. Hence, Gel:HA:PPy-NP scaffolds are a promising TE option for stimulating regeneration following nervous tissue injury.
id RCAP_fa17d3a3b74a33dddf26b7435b1ba433
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/85734
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering3D printingElectroconductive scaffoldsNeural repairPPy nanoparticlesTissue engineeringElectrically conductive bio-scaffolds are explored in the field of tissue engineering (TE) as a solution to address the clinical need of electroactive tissues, finding applications in nervous, cardiac, and spinal cord injury repair. In this work, we synthesise polypyrrole nanoparticles (PPy NP) via the mini-emulsion method with further combination with a gelatin/hyaluronic acid (HA) hydrogel to create electroconductive Gel:HA:PPy-NP TE scaffolds. Electroconductive Gel:HA:PPy-NP scaffolds possess excellent mechanical properties at 1.08 ± 0.26 MPa, closely matching the reported mechanical performance of the spinal cord. Scaffolds were designed with controlled porosity of 526.2 ± 74.6â 403.9 ± 57.4 μm, and conductivities of 4.3 à 10 â 6 ± 1.1 à 10 â 6 S.cm â 1 were reached. Rheological studies show that prior to lyophilisation, the Gel:HA:PPy-NP hydrogels display a shear-thinning behaviour. These gels were subsequently 3D printed into predefined 2 layer lattice geometries and displayed excellent post-printing shape fidelity. In vitro studies show that the Gel:HA:PPy-NP scaffolds are cytocompatible with mesenchymal stem cells and neuronal stem cells and display encouraging cell attachment and proliferation profiles. Based on these results, the incorporation of PPy NPs into Gel:HA biomaterial scaffolds enhances the conductive capabilities of the material, while showcasing biocompatible behaviour with cell cultures. Hence, Gel:HA:PPy-NP scaffolds are a promising TE option for stimulating regeneration following nervous tissue injury.The authors would like to thank the funding provided by the Irish Research Council through the Irish Research Council Enterprise Partnership Scheme with Johnson and Johnson (EPSPG/2020/78), as well as the Irish Fulbright Commission.SpringerUniversidade do MinhoSerafin, AleksandraCulebras, MarioOliveira, Joaquim M.Koffler, JacobCollins, Maurice N.2023-052023-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85734engSerafin A., Culebras M., Oliveira J. M., Koffler J., Collins M. N. 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering, Advanced Composites and Hybrid Materials , Vol. 6, Issue 109, pp. 2-14, doi:0.1007/s42114-023-00665-w, 20232522-01282522-013610.1007/s42114-023-00665-whttps://link.springer.com/content/pdf/10.1007/s42114-023-00665-w.pdf?pdf=coreinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:37:05Zoai:repositorium.sdum.uminho.pt:1822/85734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:59:06.319983Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
title 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
spellingShingle 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
Serafin, Aleksandra
3D printing
Electroconductive scaffolds
Neural repair
PPy nanoparticles
Tissue engineering
title_short 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
title_full 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
title_fullStr 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
title_full_unstemmed 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
title_sort 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering
author Serafin, Aleksandra
author_facet Serafin, Aleksandra
Culebras, Mario
Oliveira, Joaquim M.
Koffler, Jacob
Collins, Maurice N.
author_role author
author2 Culebras, Mario
Oliveira, Joaquim M.
Koffler, Jacob
Collins, Maurice N.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Serafin, Aleksandra
Culebras, Mario
Oliveira, Joaquim M.
Koffler, Jacob
Collins, Maurice N.
dc.subject.por.fl_str_mv 3D printing
Electroconductive scaffolds
Neural repair
PPy nanoparticles
Tissue engineering
topic 3D printing
Electroconductive scaffolds
Neural repair
PPy nanoparticles
Tissue engineering
description Electrically conductive bio-scaffolds are explored in the field of tissue engineering (TE) as a solution to address the clinical need of electroactive tissues, finding applications in nervous, cardiac, and spinal cord injury repair. In this work, we synthesise polypyrrole nanoparticles (PPy NP) via the mini-emulsion method with further combination with a gelatin/hyaluronic acid (HA) hydrogel to create electroconductive Gel:HA:PPy-NP TE scaffolds. Electroconductive Gel:HA:PPy-NP scaffolds possess excellent mechanical properties at 1.08 ± 0.26 MPa, closely matching the reported mechanical performance of the spinal cord. Scaffolds were designed with controlled porosity of 526.2 ± 74.6â 403.9 ± 57.4 μm, and conductivities of 4.3 à 10 â 6 ± 1.1 à 10 â 6 S.cm â 1 were reached. Rheological studies show that prior to lyophilisation, the Gel:HA:PPy-NP hydrogels display a shear-thinning behaviour. These gels were subsequently 3D printed into predefined 2 layer lattice geometries and displayed excellent post-printing shape fidelity. In vitro studies show that the Gel:HA:PPy-NP scaffolds are cytocompatible with mesenchymal stem cells and neuronal stem cells and display encouraging cell attachment and proliferation profiles. Based on these results, the incorporation of PPy NPs into Gel:HA biomaterial scaffolds enhances the conductive capabilities of the material, while showcasing biocompatible behaviour with cell cultures. Hence, Gel:HA:PPy-NP scaffolds are a promising TE option for stimulating regeneration following nervous tissue injury.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
2023-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/85734
url https://hdl.handle.net/1822/85734
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Serafin A., Culebras M., Oliveira J. M., Koffler J., Collins M. N. 3D printable electroconductive gelatin‑hyaluronic acid materials containing polypyrrole nanoparticles for electroactive tissue engineering, Advanced Composites and Hybrid Materials , Vol. 6, Issue 109, pp. 2-14, doi:0.1007/s42114-023-00665-w, 2023
2522-0128
2522-0136
10.1007/s42114-023-00665-w
https://link.springer.com/content/pdf/10.1007/s42114-023-00665-w.pdf?pdf=core
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595661352697856

Registros relacionados