Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Sónia
Data de Publicação: 2006
Outros Autores: Santos, Nuno C., Martins-Silva, J., Saldanha, Carlota
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10451/20181
Resumo: © 2006 Springer Science+Business Media, Inc.
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spelling Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effectsFibrinogenβ-estradiolDenaturationBindingFluorescence© 2006 Springer Science+Business Media, Inc.Fibrinogen is a blood plasma protein that plays a crucial role in hemostasis. It is known that erythrocyte aggregation increases in the presence of fibrinogen, and that β-estradiol decreases erythrocyte aggregation with a constant fibrinogen concentration. In this work, we have used intrinsic tryptophan fluorescence to obtain information on the conformational changes of fibrinogen upon the recently proposed interaction with β-estradiol. To evaluate the effect on the conformational changes during fibrinogen-β-estradiol binding, fluorescence experiments were performed using guanidine hydrochloride (0–6 M) as denaturant, at different pH values. The results obtained for pH 6.5 and 8.0 showed no effect during the binding. The main differences were observed between pH 4.2 and 7.4, in the absence and in the presence of two different denaturant concentrations (1 and 5 M). A red shift of the fluorescence emission from 344 to 354 nm is observed when denaturant concentration is above 3 M for all studied pH values. This phenomenon may be explained by the loss of compact structure of the protein in the presence of denaturant, with tryptophan residues exposure to the aqueous environment and alteration of fibrinogen-β-estradiol binding. These results demonstrate that the binding sites of fibrinogen are strongly dependent on the conformational state of the protein.This work was supported by the Fundação para a Ciência e Tecnologia (FCT) of the Portuguese Ministry of Science, Technology and Higher Education (MCTES).SpringerRepositório da Universidade de LisboaGonçalves, SóniaSantos, Nuno C.Martins-Silva, J.Saldanha, Carlota2015-10-01T10:23:11Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/20181engJournal of Fluorescence, Vol. 16, No. 2, March 20061053-0509http://dx.doi.org/10.1007/s10895-005-0051-ymetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:20:53Zoai:repositorio.ulisboa.pt:10451/20181Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:41:18.542753Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
title Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
spellingShingle Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
Gonçalves, Sónia
Fibrinogen
β-estradiol
Denaturation
Binding
Fluorescence
title_short Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
title_full Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
title_fullStr Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
title_full_unstemmed Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
title_sort Fibrinogen-β-estradiol binding studied by fluorescence spectroscopy : denaturation and pH effects
author Gonçalves, Sónia
author_facet Gonçalves, Sónia
Santos, Nuno C.
Martins-Silva, J.
Saldanha, Carlota
author_role author
author2 Santos, Nuno C.
Martins-Silva, J.
Saldanha, Carlota
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gonçalves, Sónia
Santos, Nuno C.
Martins-Silva, J.
Saldanha, Carlota
dc.subject.por.fl_str_mv Fibrinogen
β-estradiol
Denaturation
Binding
Fluorescence
topic Fibrinogen
β-estradiol
Denaturation
Binding
Fluorescence
description © 2006 Springer Science+Business Media, Inc.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2015-10-01T10:23:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/20181
url http://hdl.handle.net/10451/20181
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Fluorescence, Vol. 16, No. 2, March 2006
1053-0509
http://dx.doi.org/10.1007/s10895-005-0051-y
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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repository.mail.fl_str_mv info@rcaap.pt
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