Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)

Bibliographic Details
Main Author: Munlela, Benilde
Publication Date: 2020
Other Authors: João, Eva D., Donato, Celeste M., Strydom, Amy, Boene, Simone S., Chissaque, Assucênio, Bauhofer, Adilson F. L., Langa, Jerónimo, Cassocera, Marta, Cossa-Moiane, Idalécia, Chilaúle, Jorfélia J., O’Neill, Hester G., de Deus, Nilsa
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/180773
Summary: Funding Information: Funding: The study was supported by European Foundation Initiative into African Research in Neglected Tropical Diseases that awarded a senior fellowship to ND (EFINTD; 89539); World Health Organization (WHO); Deutsche Forschungsgemeinschaft (DFG; JO369/5-1) to ND and HGO which supported scholarship of BM, AC, AFLB, AS and SSB; Fundo Nacional de Investigação (FNI) fellowships to BM, AC, and JC. EDJ Ph.D. was supported by Calouste Gulbenkian Foundation. CMD is supported through the Australian National Health and Medical Research Council with an Early Career Fellowship (1113269). Funding Information: The study was supported by European Foundation Initiative into African Research in Neglected Tropical Diseases that awarded a senior fellowship to ND (EFINTD; 89539); World Health Organization (WHO); Deutsche Forschungsgemeinschaft (DFG; JO369/5-1) to ND and HGO which supported scholarship of BM, AC, AFLB, AS and SSB; Fundo Nacional de Investiga??o (FNI) fellowships to BM, AC, and JC. EDJ Ph.D. was supported by Calouste Gulbenkian Foundation. CMD is supported through the Australian National Health and Medical Research Council with an Early Career Fellowship (1113269). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
id RCAP_f59170cea482f2a614fb8cd73fe14700
oai_identifier_str oai:run.unl.pt:10362/180773
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)Bayesian analysisG1P[8]MozambiqueRotarix®Rotavirus group AWhole genome sequencingImmunology and AllergyMolecular BiologyImmunology and Microbiology(all)Microbiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: Funding: The study was supported by European Foundation Initiative into African Research in Neglected Tropical Diseases that awarded a senior fellowship to ND (EFINTD; 89539); World Health Organization (WHO); Deutsche Forschungsgemeinschaft (DFG; JO369/5-1) to ND and HGO which supported scholarship of BM, AC, AFLB, AS and SSB; Fundo Nacional de Investigação (FNI) fellowships to BM, AC, and JC. EDJ Ph.D. was supported by Calouste Gulbenkian Foundation. CMD is supported through the Australian National Health and Medical Research Council with an Early Career Fellowship (1113269). Funding Information: The study was supported by European Foundation Initiative into African Research in Neglected Tropical Diseases that awarded a senior fellowship to ND (EFINTD; 89539); World Health Organization (WHO); Deutsche Forschungsgemeinschaft (DFG; JO369/5-1) to ND and HGO which supported scholarship of BM, AC, AFLB, AS and SSB; Fundo Nacional de Investiga??o (FNI) fellowships to BM, AC, and JC. EDJ Ph.D. was supported by Calouste Gulbenkian Foundation. CMD is supported through the Australian National Health and Medical Research Council with an Early Career Fellowship (1113269). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.Mozambique introduced the Rotarix® vaccine (GSK Biologicals, Rixensart, Belgium) into the National Immunization Program in September 2015. Although G1P[8] was one of the most prevalent genotypes between 2012 and 2017 in Mozambique, no complete genomes had been sequenced to date. Here we report whole genome sequence analysis for 36 G1P[8] strains using an Illumina MiSeq platform. All strains exhibited a Wa-like genetic backbone (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). Phylogenetic analysis showed that most of the Mozambican strains clustered closely together in a conserved clade for the entire genome. No distinct clustering for pre-and post-vaccine strains were observed. These findings may suggest no selective pressure by the introduction of the Rotarix® vaccine in 2015. Two strains (HJM1646 and HGM0544) showed varied clustering for the entire genome, suggesting reassortment, whereas a further strain obtained from a rural area (MAN0033) clustered separately for all gene segments. Bayesian analysis for the VP7 and VP4 encoding gene segments supported the phylogenetic analysis and indicated a possible introduction from India around 2011.7 and 2013.0 for the main Mozambican clade. Continued monitoring of rotavirus strains in the post-vaccine period is required to fully understand the impact of vaccine introduction on the diversity and evolution of rotavirus strains.Instituto de Higiene e Medicina Tropical (IHMT)RUNMunlela, BenildeJoão, Eva D.Donato, Celeste M.Strydom, AmyBoene, Simone S.Chissaque, AssucênioBauhofer, Adilson F. L.Langa, JerónimoCassocera, MartaCossa-Moiane, IdaléciaChilaúle, Jorfélia J.O’Neill, Hester G.de Deus, Nilsa2025-03-17T21:23:37Z2020-12-062020-12-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/180773eng2076-0817PURE: 112603544https://doi.org/10.3390/pathogens9121026info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-31T02:00:58Zoai:run.unl.pt:10362/180773Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:42:03.519295Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
title Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
spellingShingle Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
Munlela, Benilde
Bayesian analysis
G1P[8]
Mozambique
Rotarix®
Rotavirus group A
Whole genome sequencing
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
title_full Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
title_fullStr Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
title_full_unstemmed Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
title_sort Whole genome characterization and evolutionary analysis of G1P[8] rotavirus a strains during the pre-and post-vaccine periods in mozambique (2012–2017)
author Munlela, Benilde
author_facet Munlela, Benilde
João, Eva D.
Donato, Celeste M.
Strydom, Amy
Boene, Simone S.
Chissaque, Assucênio
Bauhofer, Adilson F. L.
Langa, Jerónimo
Cassocera, Marta
Cossa-Moiane, Idalécia
Chilaúle, Jorfélia J.
O’Neill, Hester G.
de Deus, Nilsa
author_role author
author2 João, Eva D.
Donato, Celeste M.
Strydom, Amy
Boene, Simone S.
Chissaque, Assucênio
Bauhofer, Adilson F. L.
Langa, Jerónimo
Cassocera, Marta
Cossa-Moiane, Idalécia
Chilaúle, Jorfélia J.
O’Neill, Hester G.
de Deus, Nilsa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Munlela, Benilde
João, Eva D.
Donato, Celeste M.
Strydom, Amy
Boene, Simone S.
Chissaque, Assucênio
Bauhofer, Adilson F. L.
Langa, Jerónimo
Cassocera, Marta
Cossa-Moiane, Idalécia
Chilaúle, Jorfélia J.
O’Neill, Hester G.
de Deus, Nilsa
dc.subject.por.fl_str_mv Bayesian analysis
G1P[8]
Mozambique
Rotarix®
Rotavirus group A
Whole genome sequencing
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Bayesian analysis
G1P[8]
Mozambique
Rotarix®
Rotavirus group A
Whole genome sequencing
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: Funding: The study was supported by European Foundation Initiative into African Research in Neglected Tropical Diseases that awarded a senior fellowship to ND (EFINTD; 89539); World Health Organization (WHO); Deutsche Forschungsgemeinschaft (DFG; JO369/5-1) to ND and HGO which supported scholarship of BM, AC, AFLB, AS and SSB; Fundo Nacional de Investigação (FNI) fellowships to BM, AC, and JC. EDJ Ph.D. was supported by Calouste Gulbenkian Foundation. CMD is supported through the Australian National Health and Medical Research Council with an Early Career Fellowship (1113269). Funding Information: The study was supported by European Foundation Initiative into African Research in Neglected Tropical Diseases that awarded a senior fellowship to ND (EFINTD; 89539); World Health Organization (WHO); Deutsche Forschungsgemeinschaft (DFG; JO369/5-1) to ND and HGO which supported scholarship of BM, AC, AFLB, AS and SSB; Fundo Nacional de Investiga??o (FNI) fellowships to BM, AC, and JC. EDJ Ph.D. was supported by Calouste Gulbenkian Foundation. CMD is supported through the Australian National Health and Medical Research Council with an Early Career Fellowship (1113269). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-06
2020-12-06T00:00:00Z
2025-03-17T21:23:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/180773
url http://hdl.handle.net/10362/180773
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-0817
PURE: 112603544
https://doi.org/10.3390/pathogens9121026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833602124325322752