Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification

Bibliographic Details
Main Author: Santana, Sara D. F.
Publication Date: 2012
Other Authors: Dhadge, Vijaykumar L, Roque, Ana Cecília Afonso
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/20796
Summary: The authors thank the financial support from Fundacao para a Ciencia e a Tecnologia through Grant PEst-C/EQB/LA0006/2011 and contracts no. PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008, PTDC/EBB-BIO/118317/2010, SFRH/BD/72650/2010 for V.L.D, and Santander Totta Bank - Universidade Nova de Lisboa for the Scientific Award 2009/2010. The authors are grateful to Dr. Abid Hussain and M. Telma Barroso (REQUIMTE, FCT-UNL, Portugal) for the preparation of the synthetic affinity ligands, to Lonza Biologics, U.K. (Dr. Richard Alldread), and the Animal Cell Technology Unit of ITQB-UNL/IBET (Dr. Paula M Alves and Dr. Ana Teixeira) for providing the cells and the culture bulks and to Mr. Filipe Cardoso and Prof. Paulo Freitas (INESC-MN, Lisbon, Portugal) for the help with the VSM measurements.
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spelling Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purificationmagnetic particlesdextramimmobilizationsynthetic affinity ligandIgG purificationThe authors thank the financial support from Fundacao para a Ciencia e a Tecnologia through Grant PEst-C/EQB/LA0006/2011 and contracts no. PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008, PTDC/EBB-BIO/118317/2010, SFRH/BD/72650/2010 for V.L.D, and Santander Totta Bank - Universidade Nova de Lisboa for the Scientific Award 2009/2010. The authors are grateful to Dr. Abid Hussain and M. Telma Barroso (REQUIMTE, FCT-UNL, Portugal) for the preparation of the synthetic affinity ligands, to Lonza Biologics, U.K. (Dr. Richard Alldread), and the Animal Cell Technology Unit of ITQB-UNL/IBET (Dr. Paula M Alves and Dr. Ana Teixeira) for providing the cells and the culture bulks and to Mr. Filipe Cardoso and Prof. Paulo Freitas (INESC-MN, Lisbon, Portugal) for the help with the VSM measurements.Dextran-coated iron oxide magnetic particles modified with ligand 22/8, a protein A mimetic ligand, were prepared and assessed for IgG purification. Dextran was chosen as the agent to modify the surface of magnetic particles by presenting a negligible level of nonspecific adsorption. For the functionalization of the particles with the affinity ligand toward antibodies, three methods have been explored. The optimum coupling method yielded a theoretical maximum capacity for human IgG calculated as 568 ± 33 mg/g and a binding affinity constant of 7.7 × 10⁴ M⁻¹. Regeneration, recycle and reuse of particles was also highly successful for five cycles with minor loss of capacity. Moreover, this support presented specificity and effectiveness for IgG adsorption and elution at pH 11 directly from crude extracts with a final purity of 95% in the eluted fraction.CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)DQ - Departamento de QuímicaRUNSantana, Sara D. F.Dhadge, Vijaykumar LRoque, Ana Cecília Afonso2017-05-30T13:57:31Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/20796eng1944-8244PURE: 402419https://doi.org/10.1021/am301551ninfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:25:37Zoai:run.unl.pt:10362/20796Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:56:42.937612Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
title Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
spellingShingle Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
Santana, Sara D. F.
magnetic particles
dextram
immobilization
synthetic affinity ligand
IgG purification
title_short Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
title_full Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
title_fullStr Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
title_full_unstemmed Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
title_sort Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification
author Santana, Sara D. F.
author_facet Santana, Sara D. F.
Dhadge, Vijaykumar L
Roque, Ana Cecília Afonso
author_role author
author2 Dhadge, Vijaykumar L
Roque, Ana Cecília Afonso
author2_role author
author
dc.contributor.none.fl_str_mv CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Santana, Sara D. F.
Dhadge, Vijaykumar L
Roque, Ana Cecília Afonso
dc.subject.por.fl_str_mv magnetic particles
dextram
immobilization
synthetic affinity ligand
IgG purification
topic magnetic particles
dextram
immobilization
synthetic affinity ligand
IgG purification
description The authors thank the financial support from Fundacao para a Ciencia e a Tecnologia through Grant PEst-C/EQB/LA0006/2011 and contracts no. PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008, PTDC/EBB-BIO/118317/2010, SFRH/BD/72650/2010 for V.L.D, and Santander Totta Bank - Universidade Nova de Lisboa for the Scientific Award 2009/2010. The authors are grateful to Dr. Abid Hussain and M. Telma Barroso (REQUIMTE, FCT-UNL, Portugal) for the preparation of the synthetic affinity ligands, to Lonza Biologics, U.K. (Dr. Richard Alldread), and the Animal Cell Technology Unit of ITQB-UNL/IBET (Dr. Paula M Alves and Dr. Ana Teixeira) for providing the cells and the culture bulks and to Mr. Filipe Cardoso and Prof. Paulo Freitas (INESC-MN, Lisbon, Portugal) for the help with the VSM measurements.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2017-05-30T13:57:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/20796
url http://hdl.handle.net/10362/20796
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1944-8244
PURE: 402419
https://doi.org/10.1021/am301551n
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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