Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta

Bibliographic Details
Main Author: Rodrigo, Ana P.
Publication Date: 2022
Other Authors: Lopes, Ana Catarina, Pereira, Ricardo, Anjo, Sandra I., Manadas, Bruno, Grosso, Ana R., Baptista, Pedro V., Fernandes, Alexandra R., Costa, Pedro M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/143188
Summary: project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. The National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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oai_identifier_str oai:run.unl.pt:10362/143188
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spelling Endogenous Fluorescent Proteins in the Mucus of an Intertidal PolychaetaClues for Biotechnologyfluorescencemarine Annelidamass spectrometrymucosecretionspoisonous secretionsproteomeredox statustranscriptomicsubiquitin conjugatesUV lightDrug DiscoverySDG 3 - Good Health and Well-beingSDG 14 - Life Below Waterproject LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. The National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue–greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNRodrigo, Ana P.Lopes, Ana CatarinaPereira, RicardoAnjo, Sandra I.Manadas, BrunoGrosso, Ana R.Baptista, Pedro V.Fernandes, Alexandra R.Costa, Pedro M.2022-08-22T22:16:24Z2022-03-252022-03-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/143188eng1660-3397PURE: 43621962https://doi.org/10.3390/md20040224info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:04:29Zoai:run.unl.pt:10362/143188Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:35:19.053753Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
Clues for Biotechnology
title Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
spellingShingle Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
Rodrigo, Ana P.
fluorescence
marine Annelida
mass spectrometry
mucosecretions
poisonous secretions
proteome
redox status
transcriptomics
ubiquitin conjugates
UV light
Drug Discovery
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
title_short Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
title_full Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
title_fullStr Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
title_full_unstemmed Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
title_sort Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta
author Rodrigo, Ana P.
author_facet Rodrigo, Ana P.
Lopes, Ana Catarina
Pereira, Ricardo
Anjo, Sandra I.
Manadas, Bruno
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
author_role author
author2 Lopes, Ana Catarina
Pereira, Ricardo
Anjo, Sandra I.
Manadas, Bruno
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Rodrigo, Ana P.
Lopes, Ana Catarina
Pereira, Ricardo
Anjo, Sandra I.
Manadas, Bruno
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
dc.subject.por.fl_str_mv fluorescence
marine Annelida
mass spectrometry
mucosecretions
poisonous secretions
proteome
redox status
transcriptomics
ubiquitin conjugates
UV light
Drug Discovery
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
topic fluorescence
marine Annelida
mass spectrometry
mucosecretions
poisonous secretions
proteome
redox status
transcriptomics
ubiquitin conjugates
UV light
Drug Discovery
SDG 3 - Good Health and Well-being
SDG 14 - Life Below Water
description project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. The National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-22T22:16:24Z
2022-03-25
2022-03-25T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143188
url http://hdl.handle.net/10362/143188
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
PURE: 43621962
https://doi.org/10.3390/md20040224
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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