Tyrosinase-based phenol remediation

Bibliographic Details
Main Author: Gralheira, Daniel José Rossa
Publication Date: 2012
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/8951
Summary: Dissertation for the obtention of the Master Degree in Biotechnology
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spelling Tyrosinase-based phenol remediationBionanoconjugatesGold nanoparticleRemediationTyrosinasePhenolDissertation for the obtention of the Master Degree in BiotechnologyNanobiotechnology is an emerging area full of potentialities to explore and reshape instituted knowledge. In this work, the main goal was to design and test biological remediation systems (a total of three) taking advantage of nanotechnology features, as a step towards future progress of sense-and-shoot combined systems. This task involved the development of active bionanoconjugates (BNC) with two different tyrosinases (Agaricus bisporus - AbT, and Tricoderma reesei - TrT) to be implemented in three suggested phenol remediation systems. AuNP-MUA (gold nanoparticles capped with11-mercapto-1-undecanoic acid) 1:5000 colloid was shown to be stable up to 100 mM ionic strength (sodium cloride) and able to form active BNCs with AbT. TrT:AuNP ratio for BNC formation is recommended as 100, supported by zeta potential and agarose gel mobility experimental data which, in turn, atone with the proposed theoretical estimation. The incubation of 100 ppm phenol with BNC-MUA-AbT for 60 minutes achieved 50% substrate conversion (the efficiency of the systems were also investigated using ultravioletvisible spectrophotometry (UV-Vis) complemented by standard addition method and solid-phase microextraction - gas chromatography (SPME-GC)).Faculdade de Ciências e TecnologiaCortez, JoãoSilva, MarcoRUNGralheira, Daniel José Rossa2013-02-22T14:50:18Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/8951enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:12:36Zoai:run.unl.pt:10362/8951Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:43:25.385196Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Tyrosinase-based phenol remediation
title Tyrosinase-based phenol remediation
spellingShingle Tyrosinase-based phenol remediation
Gralheira, Daniel José Rossa
Bionanoconjugates
Gold nanoparticle
Remediation
Tyrosinase
Phenol
title_short Tyrosinase-based phenol remediation
title_full Tyrosinase-based phenol remediation
title_fullStr Tyrosinase-based phenol remediation
title_full_unstemmed Tyrosinase-based phenol remediation
title_sort Tyrosinase-based phenol remediation
author Gralheira, Daniel José Rossa
author_facet Gralheira, Daniel José Rossa
author_role author
dc.contributor.none.fl_str_mv Cortez, João
Silva, Marco
RUN
dc.contributor.author.fl_str_mv Gralheira, Daniel José Rossa
dc.subject.por.fl_str_mv Bionanoconjugates
Gold nanoparticle
Remediation
Tyrosinase
Phenol
topic Bionanoconjugates
Gold nanoparticle
Remediation
Tyrosinase
Phenol
description Dissertation for the obtention of the Master Degree in Biotechnology
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2013-02-22T14:50:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8951
url http://hdl.handle.net/10362/8951
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596144725262336

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