Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus

Bibliographic Details
Main Author: D’Ambrosio, Mariaelena
Publication Date: 2021
Other Authors: Gonçalves, Cátia, Calmão, Mariana, Rodrigues, Maria, Costa, Pedro M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/136329
Summary: FA_05_2017_007 UID/Multi/04378/2020
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spelling Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillusBioreactivesDogwhelkGastropodaMarine biotechnologyThiolsToxicityDrug DiscoverySDG 14 - Life Below WaterFA_05_2017_007 UID/Multi/04378/2020Marine biodiversity has been yielding promising novel bioproducts from venomous animals. Despite the auspices of conotoxins, which originated the paradigmatic painkiller Prialt, the biotechnological potential of gastropod venoms remains to be explored. Marine bioprospecting is expanding towards temperate species like the dogwhelk Nucella lapillus, which is suspected to secrete immobilizing agents through its salivary glands with a relaxing effect on the musculature of its preferential prey, Mytilus sp. This work focused on detecting, localizing, and testing the bioreactivity of cysteine-rich proteins and peptides, whose presence is a signature of animal venoms and poisons. The highest content of thiols was found in crude protein extracts from the digestive gland, which is associated with digestion, followed by the peribuccal mass, where the salivary glands are located. Conversely, the foot and siphon (which the gastropod uses for feeding) are not the main organs involved in toxin secretion. Ex vivo bioassays with Mytilus gill tissue disclosed the differential bioreactivity of crude protein extracts. Secretions from the digestive gland and peribuccal mass caused the most significant molecular damage, with evidence for the induction of apoptosis. These early findings indicate that salivary glands are a promising target for the extraction and characterization of bioactive cysteine-rich proteinaceous toxins from the species.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUND’Ambrosio, MariaelenaGonçalves, CátiaCalmão, MarianaRodrigues, MariaCosta, Pedro M.2022-04-12T22:48:10Z2021-05-152021-05-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/136329eng1660-3397PURE: 42960187https://doi.org/10.3390/md19050276info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:01:06Zoai:run.unl.pt:10362/136329Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:32:06.656815Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
title Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
spellingShingle Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
D’Ambrosio, Mariaelena
Bioreactives
Dogwhelk
Gastropoda
Marine biotechnology
Thiols
Toxicity
Drug Discovery
SDG 14 - Life Below Water
title_short Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
title_full Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
title_fullStr Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
title_full_unstemmed Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
title_sort Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus
author D’Ambrosio, Mariaelena
author_facet D’Ambrosio, Mariaelena
Gonçalves, Cátia
Calmão, Mariana
Rodrigues, Maria
Costa, Pedro M.
author_role author
author2 Gonçalves, Cátia
Calmão, Mariana
Rodrigues, Maria
Costa, Pedro M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv D’Ambrosio, Mariaelena
Gonçalves, Cátia
Calmão, Mariana
Rodrigues, Maria
Costa, Pedro M.
dc.subject.por.fl_str_mv Bioreactives
Dogwhelk
Gastropoda
Marine biotechnology
Thiols
Toxicity
Drug Discovery
SDG 14 - Life Below Water
topic Bioreactives
Dogwhelk
Gastropoda
Marine biotechnology
Thiols
Toxicity
Drug Discovery
SDG 14 - Life Below Water
description FA_05_2017_007 UID/Multi/04378/2020
publishDate 2021
dc.date.none.fl_str_mv 2021-05-15
2021-05-15T00:00:00Z
2022-04-12T22:48:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/136329
url http://hdl.handle.net/10362/136329
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
PURE: 42960187
https://doi.org/10.3390/md19050276
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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