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Azoreductase activity in intact cells of a non-conventional yeast strain

Bibliographic Details
Main Author: Ramalho, Patrícia Alexandra Costa Ferreira
Publication Date: 2002
Other Authors: Cardoso, M. Helena, Ramalho, Maria Teresa, Paulo, Artur Cavaco
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/2158
Summary: Poster apresentado no Annual Workshop COST 847 Textile Quality and Biotechnology, 2, Villa Olmo, Como, Itália, 10-11 Outubro 2002.
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spelling Azoreductase activity in intact cells of a non-conventional yeast strainAzoreductaseNon-conventional yeastsPoster apresentado no Annual Workshop COST 847 Textile Quality and Biotechnology, 2, Villa Olmo, Como, Itália, 10-11 Outubro 2002.Several non-conventional ascomycete yeast strains, isolated from dye-contaminated environments, display azo-reductive capabilities. Those strains can therefore decolorize azo dyes, which are widely used in the textile industries. The azoreductase activity has been described and investigated in a wide variety of anaerobic bacterial species, particularly from the intestinal microflora, and also, although more rarely, in aerobic bacteria. The present work describes some characteristics of the azoreductase present in one of the isolated yeast strains. This enzyme activity seems to be constitutive in the microorganism, as the decolourization process is not affected by pre-adaptation of the cells to the tested dyes. The azo reduction activity, measured at constant OD (optical density), has a maximum at the end of the exponential growth phase. For cells collected in this phase, the decolourization profile for the two dyes derived from N,N-dimethylaniline (I and II) presents a plateau between pH 3 and 5, while the analogue dyes derived from beta-naphtol (III and IV) have a maximum at pH 3. The azoreductase activity in resting cells shows a typical saturation kinetics in relation to the concentration of the dye. For dye II, at pH 4.0, a Km of 0,42 mM and a vmáx of 3,4 mmol*h-1*gDW-1 were obtained. Despite the low specificity of this yeast azoreductase, both the initial decolourization rate and the extent of the process are dependent on the dye structure.BIOEFTEX Project.Universidade do MinhoRamalho, Patrícia Alexandra Costa FerreiraCardoso, M. HelenaRamalho, Maria TeresaPaulo, Artur Cavaco2002-102002-10-01T00:00:00Zconference posterinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/2158enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-15T01:17:13Zoai:repositorium.sdum.uminho.pt:1822/2158Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:19:00.060034Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Azoreductase activity in intact cells of a non-conventional yeast strain
title Azoreductase activity in intact cells of a non-conventional yeast strain
spellingShingle Azoreductase activity in intact cells of a non-conventional yeast strain
Ramalho, Patrícia Alexandra Costa Ferreira
Azoreductase
Non-conventional yeasts
title_short Azoreductase activity in intact cells of a non-conventional yeast strain
title_full Azoreductase activity in intact cells of a non-conventional yeast strain
title_fullStr Azoreductase activity in intact cells of a non-conventional yeast strain
title_full_unstemmed Azoreductase activity in intact cells of a non-conventional yeast strain
title_sort Azoreductase activity in intact cells of a non-conventional yeast strain
author Ramalho, Patrícia Alexandra Costa Ferreira
author_facet Ramalho, Patrícia Alexandra Costa Ferreira
Cardoso, M. Helena
Ramalho, Maria Teresa
Paulo, Artur Cavaco
author_role author
author2 Cardoso, M. Helena
Ramalho, Maria Teresa
Paulo, Artur Cavaco
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ramalho, Patrícia Alexandra Costa Ferreira
Cardoso, M. Helena
Ramalho, Maria Teresa
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv Azoreductase
Non-conventional yeasts
topic Azoreductase
Non-conventional yeasts
description Poster apresentado no Annual Workshop COST 847 Textile Quality and Biotechnology, 2, Villa Olmo, Como, Itália, 10-11 Outubro 2002.
publishDate 2002
dc.date.none.fl_str_mv 2002-10
2002-10-01T00:00:00Z
dc.type.driver.fl_str_mv conference poster
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/2158
url https://hdl.handle.net/1822/2158
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595868966551552

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