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A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)

Bibliographic Details
Main Author: Neves, J. M.
Publication Date: 1996
Other Authors: Mota, M., Simões, N., Teixeira, J. A.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/16855
Summary: Separation of a large number of viable nematodes is essential for physiological srudies on Sleinernema carpocapsae. In this paper, we describe a fast and useful method for the separation of adults of this nematode species. This method relies on the determination of the densiry of males and females by applying a modified version of Stokes' law. Adults were separated in a discontinuous sucrase gradient at 10 and 20 %. Males and females separated by this method were 95 % viable.
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spelling A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)GradientSeparationSteinernema carpocapsaeSucroseScience & TechnologySeparation of a large number of viable nematodes is essential for physiological srudies on Sleinernema carpocapsae. In this paper, we describe a fast and useful method for the separation of adults of this nematode species. This method relies on the determination of the densiry of males and females by applying a modified version of Stokes' law. Adults were separated in a discontinuous sucrase gradient at 10 and 20 %. Males and females separated by this method were 95 % viable.Méthode rapide de séparation des adultes à partir d'une population brute de Steinernema carpocapsae (Nematoda : Steinernernatidae) - La sélection d'un grand nombre de nématodes viables est une condition impérative pour les érudes sur la physiologie de Steinernema carpocapsae. Est décrite ici une méthode rapide et pratique permenant la séparation des mâles et des femelles de ce nématode à partir d'une suspension contenant une population brute. Pour l'utilisation de cene méthode, la densité des mâles et des femelles est déterminée par application de la loi de Stokes modifiée. Les adultes sont séparés dans un gradient discontinu de saccharose s'étageant de 10 et 20 %. Quatre-vingt-quinze pour cent des mâles et des femelles isolés grâce à cene méthode restent viables.ElsevierUniversidade do MinhoNeves, J. M.Mota, M.Simões, N.Teixeira, J. A.1996-061996-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/16855eng1164-5571info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T05:18:31Zoai:repositorium.sdum.uminho.pt:1822/16855Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:22:06.270001Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
title A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
spellingShingle A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
Neves, J. M.
Gradient
Separation
Steinernema carpocapsae
Sucrose
Science & Technology
title_short A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
title_full A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
title_fullStr A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
title_full_unstemmed A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
title_sort A rapid method for separation of adults in a mixed population of Steinernema carpocapsae (Nematoda : Steinernematidae)
author Neves, J. M.
author_facet Neves, J. M.
Mota, M.
Simões, N.
Teixeira, J. A.
author_role author
author2 Mota, M.
Simões, N.
Teixeira, J. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Neves, J. M.
Mota, M.
Simões, N.
Teixeira, J. A.
dc.subject.por.fl_str_mv Gradient
Separation
Steinernema carpocapsae
Sucrose
Science & Technology
topic Gradient
Separation
Steinernema carpocapsae
Sucrose
Science & Technology
description Separation of a large number of viable nematodes is essential for physiological srudies on Sleinernema carpocapsae. In this paper, we describe a fast and useful method for the separation of adults of this nematode species. This method relies on the determination of the densiry of males and females by applying a modified version of Stokes' law. Adults were separated in a discontinuous sucrase gradient at 10 and 20 %. Males and females separated by this method were 95 % viable.
publishDate 1996
dc.date.none.fl_str_mv 1996-06
1996-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/16855
url https://hdl.handle.net/1822/16855
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1164-5571
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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