Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation

Bibliographic Details
Main Author: Vilela-Alves, Guilherme
Publication Date: 2022
Other Authors: Manuel, Rita Rebelo, Oliveira, Ana Rita, Pereira, Inês Cardoso, Romão, Maria João, Mota, Cristiano
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/150787
Summary: Publisher Copyright: © 2022 by the authors.
id RCAP_ef07a3cf4267469aa57972e3772f33af
oai_identifier_str oai:run.unl.pt:10362/150787
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme ReoxidationCO reductionformate dehydrogenasemolybdopterinredox enzymestungsten cofactorX-ray crystallographyCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 13 - Climate ActionPublisher Copyright: © 2022 by the authors.Metal-dependent formate dehydrogenases (Fdh) catalyze the reversible conversion of CO2 to formate, with unrivalled efficiency and selectivity. However, the key catalytic aspects of these enzymes remain unknown, preventing us from fully benefiting from their capabilities in terms of biotechnological applications. Here, we report a time-resolved characterization by X-ray crystallography of the Desulfovibrio vulgaris Hildenborough SeCys/W-Fdh during formate oxidation. The results allowed us to model five different intermediate structures and to chronologically map the changes occurring during enzyme reduction. Formate molecules were assigned for the first time to populate the catalytic pocket of a Fdh. Finally, the redox reversibility of DvFdhAB in crystals was confirmed by reduction and reoxidation structural studies.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNVilela-Alves, GuilhermeManuel, Rita RebeloOliveira, Ana RitaPereira, Inês CardosoRomão, Maria JoãoMota, Cristiano2023-03-17T22:26:19Z2022-12-282022-12-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/150787eng1661-6596PURE: 55678411https://doi.org/10.3390/ijms24010476info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:10:14Zoai:run.unl.pt:10362/150787Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:40:47.077494Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
title Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
spellingShingle Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
Vilela-Alves, Guilherme
CO reduction
formate dehydrogenase
molybdopterin
redox enzymes
tungsten cofactor
X-ray crystallography
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 13 - Climate Action
title_short Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
title_full Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
title_fullStr Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
title_full_unstemmed Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
title_sort Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation
author Vilela-Alves, Guilherme
author_facet Vilela-Alves, Guilherme
Manuel, Rita Rebelo
Oliveira, Ana Rita
Pereira, Inês Cardoso
Romão, Maria João
Mota, Cristiano
author_role author
author2 Manuel, Rita Rebelo
Oliveira, Ana Rita
Pereira, Inês Cardoso
Romão, Maria João
Mota, Cristiano
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Vilela-Alves, Guilherme
Manuel, Rita Rebelo
Oliveira, Ana Rita
Pereira, Inês Cardoso
Romão, Maria João
Mota, Cristiano
dc.subject.por.fl_str_mv CO reduction
formate dehydrogenase
molybdopterin
redox enzymes
tungsten cofactor
X-ray crystallography
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 13 - Climate Action
topic CO reduction
formate dehydrogenase
molybdopterin
redox enzymes
tungsten cofactor
X-ray crystallography
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 13 - Climate Action
description Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-28
2022-12-28T00:00:00Z
2023-03-17T22:26:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150787
url http://hdl.handle.net/10362/150787
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 55678411
https://doi.org/10.3390/ijms24010476
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596882290475008

Similar Items