Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

Bibliographic Details
Main Author: Almeida, Juliana R.
Publication Date: 2022
Other Authors: Fradinho, Joana C., Carvalho, Gilda, Oehmen, Adrian, Reis, Maria A. M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/133196
Summary: DFA/BD/8201/2020 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating CulturesDNA sequencingDomestic wastewaterFeast and famine (FF)Fluorescence in situ hybridization (FISH)Microbial communitiesPermanent feast (PF)Phototrophic mixed cultures (PMC)Phototrophic purple bacteria (PPB)Polyhydroxyalkanoates (PHA)MicrobiologyMicrobiology (medical)VirologyDFA/BD/8201/2020 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020Phototrophic mixed cultures (PMC) are versatile systems which can be applied for waste streams, valorisation and production of added-value compounds, such as polyhydroxyalkanoates (PHA). This work evaluates the influence of different operational conditions on the bacterial communities reported in PMC systems with PHA production capabilities. Eleven PMCs, fed either with acetate or fermented wastewater, and selected under either feast and famine (FF) or permanent feast (PF) regimes, were evaluated. Overall, results identified Chromatiaceae members as the main phototrophic PHA producers, along with Rhodopseudomonas, Rhodobacter and Rhizobium. The findings show that Chromatiaceae were favoured under operating conditions with high carbon concentrations, and particularly under the PF regime. In FF systems fed with fermented wastewater, the results indicate that increasing the organic loading rate enriches for Rhodopseudomonas, Rhizobium and Hyphomicrobiaceae, which together with Rhodobacter and Chromatiaceae, were likely responsible for PHA storage. In addition, high-sugar feedstock impairs PHA production under PF conditions (fermentative bacteria dominance), which does not occur under FF. This characterization of the communities responsible for PHA accumulation helps to define improved operational strategies for PHA production with PMC.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNAlmeida, Juliana R.Fradinho, Joana C.Carvalho, GildaOehmen, AdrianReis, Maria A. M.2022-02-18T23:19:06Z2022-02-032022-02-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133196eng2076-2607PURE: 36690330https://doi.org/10.3390/microorganisms10020351info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:59:37Zoai:run.unl.pt:10362/133196Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:30:38.351886Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
spellingShingle Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
Almeida, Juliana R.
DNA sequencing
Domestic wastewater
Feast and famine (FF)
Fluorescence in situ hybridization (FISH)
Microbial communities
Permanent feast (PF)
Phototrophic mixed cultures (PMC)
Phototrophic purple bacteria (PPB)
Polyhydroxyalkanoates (PHA)
Microbiology
Microbiology (medical)
Virology
title_short Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_full Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_fullStr Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_full_unstemmed Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_sort Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
author Almeida, Juliana R.
author_facet Almeida, Juliana R.
Fradinho, Joana C.
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
author_role author
author2 Fradinho, Joana C.
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Almeida, Juliana R.
Fradinho, Joana C.
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
dc.subject.por.fl_str_mv DNA sequencing
Domestic wastewater
Feast and famine (FF)
Fluorescence in situ hybridization (FISH)
Microbial communities
Permanent feast (PF)
Phototrophic mixed cultures (PMC)
Phototrophic purple bacteria (PPB)
Polyhydroxyalkanoates (PHA)
Microbiology
Microbiology (medical)
Virology
topic DNA sequencing
Domestic wastewater
Feast and famine (FF)
Fluorescence in situ hybridization (FISH)
Microbial communities
Permanent feast (PF)
Phototrophic mixed cultures (PMC)
Phototrophic purple bacteria (PPB)
Polyhydroxyalkanoates (PHA)
Microbiology
Microbiology (medical)
Virology
description DFA/BD/8201/2020 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020
publishDate 2022
dc.date.none.fl_str_mv 2022-02-18T23:19:06Z
2022-02-03
2022-02-03T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/133196
url http://hdl.handle.net/10362/133196
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 36690330
https://doi.org/10.3390/microorganisms10020351
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596745320235008

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