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Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification

Bibliographic Details
Main Author: Coelho, Beatriz J.
Publication Date: 2023
Other Authors: Neto, Joana P., Sieira, Bárbara, Moura, André T., Fortunato, Elvira, Martins, Rodrigo, Baptista, Pedro V., Igreja, Rui, Águas, Hugo
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/154845
Summary: Publisher Copyright: © 2023 by the authors.
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spelling Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid AmplificationDesign, Fabrication and Characterizationdigital microfluidicsdroplet microfluidicsflow focusingnegative pressureAnalytical ChemistryInformation SystemsAtomic and Molecular Physics, and OpticsBiochemistryInstrumentationElectrical and Electronic EngineeringPublisher Copyright: © 2023 by the authors.Microfluidic-based platforms have become a hallmark for chemical and biological assays, empowering micro- and nano-reaction vessels. The fusion of microfluidic technologies (digital microfluidics, continuous-flow microfluidics, and droplet microfluidics, just to name a few) presents great potential for overcoming the inherent limitations of each approach, while also elevating their respective strengths. This work exploits the combination of digital microfluidics (DMF) and droplet microfluidics (DrMF) on a single substrate, where DMF enables droplet mixing and further acts as a controlled liquid supplier for a high-throughput nano-liter droplet generator. Droplet generation is performed at a flow-focusing region, operating on dual pressure: negative pressure applied to the aqueous phase and positive pressure applied to the oil phase. We evaluate the droplets produced with our hybrid DMF–DrMF devices in terms of droplet volume, speed, and production frequency and further compare them with standalone DrMF devices. Both types of devices enable customizable droplet production (various volumes and circulation speeds), yet hybrid DMF–DrMF devices yield more controlled droplet production while achieving throughputs that are similar to standalone DrMF devices. These hybrid devices enable the production of up to four droplets per second, which reach a maximum circulation speed close to 1540 µm/s and volumes as low as 0.5 nL.DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNCoelho, Beatriz J.Neto, Joana P.Sieira, BárbaraMoura, André T.Fortunato, ElviraMartins, RodrigoBaptista, Pedro V.Igreja, RuiÁguas, Hugo2023-07-04T22:17:54Z2023-05-202023-05-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/154845eng1424-8220PURE: 65211841https://doi.org/10.3390/s23104927info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:12:38Zoai:run.unl.pt:10362/154845Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:42:54.253266Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
Design, Fabrication and Characterization
title Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
spellingShingle Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
Coelho, Beatriz J.
digital microfluidics
droplet microfluidics
flow focusing
negative pressure
Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
title_short Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
title_full Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
title_fullStr Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
title_full_unstemmed Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
title_sort Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification
author Coelho, Beatriz J.
author_facet Coelho, Beatriz J.
Neto, Joana P.
Sieira, Bárbara
Moura, André T.
Fortunato, Elvira
Martins, Rodrigo
Baptista, Pedro V.
Igreja, Rui
Águas, Hugo
author_role author
author2 Neto, Joana P.
Sieira, Bárbara
Moura, André T.
Fortunato, Elvira
Martins, Rodrigo
Baptista, Pedro V.
Igreja, Rui
Águas, Hugo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCM - Departamento de Ciência dos Materiais
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
RUN
dc.contributor.author.fl_str_mv Coelho, Beatriz J.
Neto, Joana P.
Sieira, Bárbara
Moura, André T.
Fortunato, Elvira
Martins, Rodrigo
Baptista, Pedro V.
Igreja, Rui
Águas, Hugo
dc.subject.por.fl_str_mv digital microfluidics
droplet microfluidics
flow focusing
negative pressure
Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
topic digital microfluidics
droplet microfluidics
flow focusing
negative pressure
Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
description Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-04T22:17:54Z
2023-05-20
2023-05-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/154845
url http://hdl.handle.net/10362/154845
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1424-8220
PURE: 65211841
https://doi.org/10.3390/s23104927
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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