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Amperometric measurements of nitric oxide in erythrocytes

Bibliographic Details
Main Author: Carvalho, Filomena A.
Publication Date: 2004
Other Authors: Martins-Silva, João, Carlota, Saldanha
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/17629
Summary: © 2004 Elsevier B.V. All rights reserved.
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spelling Amperometric measurements of nitric oxide in erythrocytesNitric oxideNitric oxide sensorAmperometric methodErythrocytes© 2004 Elsevier B.V. All rights reserved.In the recent years, there has been an increase in the development of new biosensors that could be helpful in the study of various physiological processes. In this study, we report the development of a new in vitro experimental design for real-time nitric oxide (NO) amperometric measurements in erythrocyte suspensions. To achieve this, we employed human erythrocyte suspensions in sodium chloride 0.9%, pH 7 (haematocrit 0.05%). The production of NO by erythrocytes was measured with a commercial NO sensor during stimulation by l-arginine, acetylcholine, choline, atropine and velnacrine maleate (10 μM of final concentrations). We also measured the nitrite and nitrate concentrations produced by erythrocyte suspensions stimulated with the above effectors by means of the Griess reaction method. We observed that there was a direct relation between the electric current produced by the NO sensor, and the NO standard concentrations, thereby leading to a good calibration curve. The in vitro erythrocytes produced significant amperometric NO values in response to a wide range of effectors and these results have the same variation profile of the nitrites and nitrates results achieved with the Griess method. In conclusion, the amperometric NO sensor constitutes a reliable method for direct, and real-time measurement in vitro of the NO production of erythrocyte suspensions, As such, it offers a potential diagnostic technique for the evaluation of diseases, and the therapeutic progression of diseases, related to intracellular NO metabolism.This work was supported by the Faculdade de Medicina da Universidade de Lisboa and by the Fundação para a Ciência e Tecnologia, Ministério da Ciência e Tecnologia, Lisboa, Portugal.ElsevierRepositório da Universidade de LisboaCarvalho, Filomena A.Martins-Silva, JoãoCarlota, Saldanha2015-03-05T14:30:59Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17629engBiosensors and Bioelectronics 20 (2004) 505–5080956-5663http://dx.doi.org/10.1016/j.bios.2004.02.015metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:17:25Zoai:repositorio.ulisboa.pt:10451/17629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:39:53.386152Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Amperometric measurements of nitric oxide in erythrocytes
title Amperometric measurements of nitric oxide in erythrocytes
spellingShingle Amperometric measurements of nitric oxide in erythrocytes
Carvalho, Filomena A.
Nitric oxide
Nitric oxide sensor
Amperometric method
Erythrocytes
title_short Amperometric measurements of nitric oxide in erythrocytes
title_full Amperometric measurements of nitric oxide in erythrocytes
title_fullStr Amperometric measurements of nitric oxide in erythrocytes
title_full_unstemmed Amperometric measurements of nitric oxide in erythrocytes
title_sort Amperometric measurements of nitric oxide in erythrocytes
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Martins-Silva, João
Carlota, Saldanha
author_role author
author2 Martins-Silva, João
Carlota, Saldanha
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Martins-Silva, João
Carlota, Saldanha
dc.subject.por.fl_str_mv Nitric oxide
Nitric oxide sensor
Amperometric method
Erythrocytes
topic Nitric oxide
Nitric oxide sensor
Amperometric method
Erythrocytes
description © 2004 Elsevier B.V. All rights reserved.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2015-03-05T14:30:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/17629
url http://hdl.handle.net/10451/17629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biosensors and Bioelectronics 20 (2004) 505–508
0956-5663
http://dx.doi.org/10.1016/j.bios.2004.02.015
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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