Evaluating aflatoxin gene expression in Aspergillus section Flavi

Bibliographic Details
Main Author: Rodrigues, Paula
Publication Date: 2017
Other Authors: Gil-Serna, Jéssica, González-Jaén, M. Teresa
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10198/13739
Summary: The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.
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spelling Evaluating aflatoxin gene expression in Aspergillus section FlaviThe determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.SpringerBiblioteca Digital do IPBRodrigues, PaulaGil-Serna, JéssicaGonzález-Jaén, M. Teresa2017-01-12T11:43:24Z20172017-01-01T00:00:00Zbook partinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10198/13739engRodrigues, Paula; Gil-Serna, Jéssica; González-Jaén, M. Teresa (2016). Evaluating aflatoxin gene expression in Aspergillus section Flavi. In Antonio Moretti, Antonia Susca, Eds. Mycotoxigenic Fungi, Methods and Protocols. New York: Springer. Part II, Vol. 1542, Chapter 15, p. 237–247. ISBN 978-1-4939-6705-6978-1-4939-6705-610.1007/978-1-4939-6707-0_15info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-25T12:04:10Zoai:bibliotecadigital.ipb.pt:10198/13739Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T11:30:32.593478Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Evaluating aflatoxin gene expression in Aspergillus section Flavi
title Evaluating aflatoxin gene expression in Aspergillus section Flavi
spellingShingle Evaluating aflatoxin gene expression in Aspergillus section Flavi
Rodrigues, Paula
title_short Evaluating aflatoxin gene expression in Aspergillus section Flavi
title_full Evaluating aflatoxin gene expression in Aspergillus section Flavi
title_fullStr Evaluating aflatoxin gene expression in Aspergillus section Flavi
title_full_unstemmed Evaluating aflatoxin gene expression in Aspergillus section Flavi
title_sort Evaluating aflatoxin gene expression in Aspergillus section Flavi
author Rodrigues, Paula
author_facet Rodrigues, Paula
Gil-Serna, Jéssica
González-Jaén, M. Teresa
author_role author
author2 Gil-Serna, Jéssica
González-Jaén, M. Teresa
author2_role author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Rodrigues, Paula
Gil-Serna, Jéssica
González-Jaén, M. Teresa
description The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-12T11:43:24Z
2017
2017-01-01T00:00:00Z
dc.type.driver.fl_str_mv book part
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/13739
url http://hdl.handle.net/10198/13739
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Paula; Gil-Serna, Jéssica; González-Jaén, M. Teresa (2016). Evaluating aflatoxin gene expression in Aspergillus section Flavi. In Antonio Moretti, Antonia Susca, Eds. Mycotoxigenic Fungi, Methods and Protocols. New York: Springer. Part II, Vol. 1542, Chapter 15, p. 237–247. ISBN 978-1-4939-6705-6
978-1-4939-6705-6
10.1007/978-1-4939-6707-0_15
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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