Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774

Bibliographic Details
Main Author: Moura, Isabel
Publication Date: 2003
Other Authors: Cunha, Carlos A., Macieira, Sofia, Dias, João M., Almeida, Gabriela Machado de, Gonçalves, Luisa L., Costa, Cristina, Lampreia, Jorge, Huber, Robert, Moura, José J. G., Romão, Maria J.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/1658
Summary: The Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003
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spelling Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774The relevance of the two calcium sites inthe structure of the catalytic subunit (NrfA)The Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003The gene encoding cytochrome c nitrite reductase(NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium site with octahedral geometry, coordinated to propionates of hemes 3 and 4, and caged by a loop non-existent in the previous structures. The highly negative electrostatic potential in the environment around hemes 3 and 4 suggests that the main role of this calcium ion may not be electrostatic but structural, namely in the stabilization of the conformation of the additional loop that cages it and influences the solvent accessibility of heme 4. The NrfA active site is similar to that of peroxidases with a nearby calcium site at the heme distal side nearly in the same location as occurs in the class II and class III peroxidases. This fact suggests that the calcium ion at the distal side of the active site in the NrfA enzymes may have a similar physiological role to that reported for the peroxidases.ASBMBRUNMoura, IsabelCunha, Carlos A.Macieira, SofiaDias, João M.Almeida, Gabriela Machado deGonçalves, Luisa L.Costa, CristinaLampreia, JorgeHuber, RobertMoura, José J. G.Romão, Maria J.2008-09-23T14:33:20Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1658enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:06:18Zoai:run.unl.pt:10362/1658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:37:10.385394Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
The relevance of the two calcium sites inthe structure of the catalytic subunit (NrfA)
title Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
spellingShingle Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
Moura, Isabel
title_short Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
title_full Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
title_fullStr Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
title_full_unstemmed Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
title_sort Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
author Moura, Isabel
author_facet Moura, Isabel
Cunha, Carlos A.
Macieira, Sofia
Dias, João M.
Almeida, Gabriela Machado de
Gonçalves, Luisa L.
Costa, Cristina
Lampreia, Jorge
Huber, Robert
Moura, José J. G.
Romão, Maria J.
author_role author
author2 Cunha, Carlos A.
Macieira, Sofia
Dias, João M.
Almeida, Gabriela Machado de
Gonçalves, Luisa L.
Costa, Cristina
Lampreia, Jorge
Huber, Robert
Moura, José J. G.
Romão, Maria J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Moura, Isabel
Cunha, Carlos A.
Macieira, Sofia
Dias, João M.
Almeida, Gabriela Machado de
Gonçalves, Luisa L.
Costa, Cristina
Lampreia, Jorge
Huber, Robert
Moura, José J. G.
Romão, Maria J.
description The Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2008-09-23T14:33:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1658
url http://hdl.handle.net/10362/1658
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ASBMB
publisher.none.fl_str_mv ASBMB
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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