S. aureus modulates P. aeruginosa small-colony variants formation

Bibliographic Details
Main Author: Magalhães, Andreia Patrícia
Publication Date: 2017
Other Authors: Sousa, Ana Margarida Martins, Lopes, Susana Patrícia, Pereira, Maria Olívia
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/47189
Summary: EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling S. aureus modulates P. aeruginosa small-colony variants formationCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Biotecnologia IndustrialEUROBIOFILMS 2017 - 5th European Congress on Microbial BiofilmsSome studies reported that, in polymicrobial consortia, some P. a eruginosa exoproducts suppresses the growth of S.aureus and provokes the emergence of SCV, associated with antimicrobial resistance, altered metabolism and reduced immunogenicity. However, the role of S.aureus in P.aerugionsa behavior has still not been deciphered. This work aimed to deeply investigate the phenotypic changes undergone by P. aeruginosa and S.aureus in a co-infection scenario. The cells recovered from 24 -h-old single and dual-species biofilms were inspected regarding their colony morphology differentiation, antibiotic susceptibility profiles and expression of virulence factors. The population analysis profiles showed the existence of two P.aeruginosa SCV resulting only from the dual-species biofilms. These SCV exhibited impressive ability to form biofilm, impaired swimming, twitching and swarming abilities, in comparison with the wild -type morphotype. Their morphologies remained unchangeable over 10 passages onto solid media, which may mean these phenotypic alterations were not adaptive. Yet, the two SCV were susceptible to the action of several classes of antibiotics. In conclusion, the presence of S.aureus in biofilms seemed to modulate some phenotypic alterations in P.aeruginosa that may be clinically relevant as two SCV were detected. As t he role of S. aureus over P. aeruginosa stills not full clear, some tests are being run to more comprehensively know how these P. aeruginosa SCV modulate the dual -species community response to in - use antimicrobials.Portuguese Foundation for Science and Technology (FCT), through the strategic funding of UID/BIO/04469/2013 and COMPETE 2020 (POCI- 01-0145-FEDER-006684), and FCT and the European Community fund FEDER, through COMPETE and BioTecNorte operation (NORTE-01-0145-FEDER -000004) funded by the European Regional Development Fund under the scope of Norte2020. Grants of SPL (SFRH/BPD/95616/2013) and APM (UMINHO/BD/25/2016)info:eu-repo/semantics/publishedVersionEuropean Society of Clinical Microbiology and Infectious Diseases (ESCMID)Universidade do MinhoMagalhães, Andreia PatríciaSousa, Ana Margarida MartinsLopes, Susana PatríciaPereira, Maria Olívia2017-09-192017-09-19T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/47189engMagalhães, Andreia P.; Sousa, Ana Margarida; Lopes, Susana P.; Pereira, Maria Olívia, S. aureus modulates P. aeruginosa small-colony variants formation. EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms. No. 004, Amesterdam, Netherlands, 19-22 September, 79, 2017.http://www.eurobiofilms.com/info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:57:19Zoai:repositorium.sdum.uminho.pt:1822/47189Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:10:03.920255Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv S. aureus modulates P. aeruginosa small-colony variants formation
title S. aureus modulates P. aeruginosa small-colony variants formation
spellingShingle S. aureus modulates P. aeruginosa small-colony variants formation
Magalhães, Andreia Patrícia
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
title_short S. aureus modulates P. aeruginosa small-colony variants formation
title_full S. aureus modulates P. aeruginosa small-colony variants formation
title_fullStr S. aureus modulates P. aeruginosa small-colony variants formation
title_full_unstemmed S. aureus modulates P. aeruginosa small-colony variants formation
title_sort S. aureus modulates P. aeruginosa small-colony variants formation
author Magalhães, Andreia Patrícia
author_facet Magalhães, Andreia Patrícia
Sousa, Ana Margarida Martins
Lopes, Susana Patrícia
Pereira, Maria Olívia
author_role author
author2 Sousa, Ana Margarida Martins
Lopes, Susana Patrícia
Pereira, Maria Olívia
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Magalhães, Andreia Patrícia
Sousa, Ana Margarida Martins
Lopes, Susana Patrícia
Pereira, Maria Olívia
dc.subject.por.fl_str_mv Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
topic Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
description EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms
publishDate 2017
dc.date.none.fl_str_mv 2017-09-19
2017-09-19T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/47189
url http://hdl.handle.net/1822/47189
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Magalhães, Andreia P.; Sousa, Ana Margarida; Lopes, Susana P.; Pereira, Maria Olívia, S. aureus modulates P. aeruginosa small-colony variants formation. EUROBIOFILMS 2017 - 5th European Congress on Microbial Biofilms. No. 004, Amesterdam, Netherlands, 19-22 September, 79, 2017.
http://www.eurobiofilms.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv European Society of Clinical Microbiology and Infectious Diseases (ESCMID)
publisher.none.fl_str_mv European Society of Clinical Microbiology and Infectious Diseases (ESCMID)
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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