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Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen

Bibliographic Details
Main Author: Moreira, Pedro Miguel Lopes
Publication Date: 2014
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/13012
Summary: Dissertação para a obtenção do grau de Mestre em Genética Molecular e Biomedicina
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spelling Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleenNotch signaling – Delta1OrganogenesisThymusSpleenHematopoietic progenitorsB cellsDissertação para a obtenção do grau de Mestre em Genética Molecular e BiomedicinaThe lymphoid organs, thymus and spleen, are essential for the differentiation of T and B cells, respectively. One of the signaling pathways involved in processes of cell differentiation is the Notch signaling pathway. Work conducted by our group demonstrated that Notch ligands, receptors and target genes are expressed in the thymic epithelium. In this work, we aimed: 1) to study the organogenesis of the thymus and spleen in chicken embryos; 2) phenotypically characterize the cells present in both organs; 3) to study the role of Notch signaling in the differentiation of hematopoietic progenitors (PH) isolated from thymus and spleen of the chicken embryo. To study the formation of the thymus and spleen and characterize the cell populations present within them during embryonic development, we stained histological sections of the organs and citospins with Hematoxylin-Eosin (HE). To elucidate the role of Notch signaling in the differentiation of the PH, we used a co-culture system of PH with stromal cells that express specific Notch ligands, Delta 1 (Dl1) and Delta 4 (Dl4). The results confirm that, the spleen is an early (E6) vascularized organ and the number of PH in the spleen increases after day E10. In the thymus, the regionalization in cortex and medulla occurs at day E8 and there are no visible neural crest cells during the several embryonic stages studied. We conclude that hematopoietic cells collected from the thymus at day E15 are more differentiated than cells collected from thymus E13. The co-cultures results demonstrated that the Notch ligand Dl1 appears to favor the lymphoid progenitor cells (LPC) proliferation/survival. Thus, the Dl1 ligand may have an important role at the early stages of T cell differentiation. Moreover, these evidences seem to confirm that the undifferentiated LPCs are able to respond more effectively to external stimulus and differentiate into T cells more efficiently.Faculdade de Ciências e TecnologiaHenriques, IsabelZilhão, RitaRUNMoreira, Pedro Miguel Lopes2014-07-29T10:27:56Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/13012enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:16:41Zoai:run.unl.pt:10362/13012Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:47:28.100425Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
title Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
spellingShingle Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
Moreira, Pedro Miguel Lopes
Notch signaling – Delta1
Organogenesis
Thymus
Spleen
Hematopoietic progenitors
B cells
title_short Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
title_full Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
title_fullStr Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
title_full_unstemmed Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
title_sort Role of Notch signaling on the differentiation of early lymphoid progenitors cells: a view throughout the development of the embryonic chicken thymus and spleen
author Moreira, Pedro Miguel Lopes
author_facet Moreira, Pedro Miguel Lopes
author_role author
dc.contributor.none.fl_str_mv Henriques, Isabel
Zilhão, Rita
RUN
dc.contributor.author.fl_str_mv Moreira, Pedro Miguel Lopes
dc.subject.por.fl_str_mv Notch signaling – Delta1
Organogenesis
Thymus
Spleen
Hematopoietic progenitors
B cells
topic Notch signaling – Delta1
Organogenesis
Thymus
Spleen
Hematopoietic progenitors
B cells
description Dissertação para a obtenção do grau de Mestre em Genética Molecular e Biomedicina
publishDate 2014
dc.date.none.fl_str_mv 2014-07-29T10:27:56Z
2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/13012
url http://hdl.handle.net/10362/13012
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596194595536896

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