Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Texto Completo: | http://hdl.handle.net/10362/180933 |
Resumo: | Funding Information: Funding: This research was funded by Fundação para a Ciência e Tecnologia (FCT) under the project TickOmic (PTDC/CVT-CVT/29073/2017). JC and JF are the recipients of Ph.D. grants supported by the FCT (SFRH/BD/121946/2016, SFRH/BD/122894/2016, respectively). Funding Information: Acknowledgments: A special thanks to Dr. Abel Oliva (Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa) who kindly provided the Israeli B. ovis strain inoculum. Authors would like to acknowledge FCT for funding the R&D Centre Global Health and Tropical Medicine (GHTM, UID/Multi/04413/2013). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
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Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursaTicksBabesiaProteomicRNAiUB2NPCCAMicrobiologyMolecular BiologySDG 3 - Good Health and Well-beingFunding Information: Funding: This research was funded by Fundação para a Ciência e Tecnologia (FCT) under the project TickOmic (PTDC/CVT-CVT/29073/2017). JC and JF are the recipients of Ph.D. grants supported by the FCT (SFRH/BD/121946/2016, SFRH/BD/122894/2016, respectively). Funding Information: Acknowledgments: A special thanks to Dr. Abel Oliva (Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa) who kindly provided the Israeli B. ovis strain inoculum. Authors would like to acknowledge FCT for funding the R&D Centre Global Health and Tropical Medicine (GHTM, UID/Multi/04413/2013). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.The negative impact of ticks and tick-borne diseases on animals and human health is driving research to discover novel targets affecting both vectors and pathogens. The salivary glands are involved in feeding and pathogen transmission, thus are considered as a compelling target to focus research. In this study, proteomics approach was used to characterize Rhipicephalus bursa sialoproteome in response to Babesia ovis infection and blood feeding. Two potential tick protective antigens were identified and its influence in tick biological parameters and pathogen infection was evaluated. Results demonstrate that the R. bursa sialoproteome is highly affected by feeding but infection is well tolerated by tick cells. The combination of both stimuli shifts the previous scenario and a more evident pathogen manipulation can be suggested. Knockdown of ub2n led to a significative increase of infection in tick salivary glands but a brusque decrease in the progeny, revealing its importance in the cellular response to pathogen infection, which is worth pursuing in future studies. Additionally, an impact in the recovery rate of adults (62%), the egg production efficiency (45.75%), and the hatching rate (88.57 %) was detected. Building knowledge on vector and/or pathogen interplay bridges the identification of protective antigens and the development of novel control strategies.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNCouto, JoanaVillar, MargaritaMateos-Hernández, LourdesFerrolho, JoanaSanches, Gustavo S.Santos, Ana SofiaSantos-Silva, Maria MargaridaNobre, JoãoMoreira, OlgaAntunes, SandraFuente, José de laDomingos, Ana2025-03-19T21:06:18Z2020-02-192020-02-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/10362/180933engPURE: 17192368https://doi.org/10.3390/vaccines8010091info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-31T02:02:22Zoai:run.unl.pt:10362/180933Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:42:09.762096Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| title |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| spellingShingle |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa Couto, Joana Ticks Babesia Proteomic RNAi UB2N PCCA Microbiology Molecular Biology SDG 3 - Good Health and Well-being |
| title_short |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| title_full |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| title_fullStr |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| title_full_unstemmed |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| title_sort |
Quantitative proteomics identifies metabolic pathways affected by babesia infection and blood feeding in the sialoproteome of the vector Rhipicephalus bursa |
| author |
Couto, Joana |
| author_facet |
Couto, Joana Villar, Margarita Mateos-Hernández, Lourdes Ferrolho, Joana Sanches, Gustavo S. Santos, Ana Sofia Santos-Silva, Maria Margarida Nobre, João Moreira, Olga Antunes, Sandra Fuente, José de la Domingos, Ana |
| author_role |
author |
| author2 |
Villar, Margarita Mateos-Hernández, Lourdes Ferrolho, Joana Sanches, Gustavo S. Santos, Ana Sofia Santos-Silva, Maria Margarida Nobre, João Moreira, Olga Antunes, Sandra Fuente, José de la Domingos, Ana |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) RUN |
| dc.contributor.author.fl_str_mv |
Couto, Joana Villar, Margarita Mateos-Hernández, Lourdes Ferrolho, Joana Sanches, Gustavo S. Santos, Ana Sofia Santos-Silva, Maria Margarida Nobre, João Moreira, Olga Antunes, Sandra Fuente, José de la Domingos, Ana |
| dc.subject.por.fl_str_mv |
Ticks Babesia Proteomic RNAi UB2N PCCA Microbiology Molecular Biology SDG 3 - Good Health and Well-being |
| topic |
Ticks Babesia Proteomic RNAi UB2N PCCA Microbiology Molecular Biology SDG 3 - Good Health and Well-being |
| description |
Funding Information: Funding: This research was funded by Fundação para a Ciência e Tecnologia (FCT) under the project TickOmic (PTDC/CVT-CVT/29073/2017). JC and JF are the recipients of Ph.D. grants supported by the FCT (SFRH/BD/121946/2016, SFRH/BD/122894/2016, respectively). Funding Information: Acknowledgments: A special thanks to Dr. Abel Oliva (Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa) who kindly provided the Israeli B. ovis strain inoculum. Authors would like to acknowledge FCT for funding the R&D Centre Global Health and Tropical Medicine (GHTM, UID/Multi/04413/2013). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-02-19 2020-02-19T00:00:00Z 2025-03-19T21:06:18Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/180933 |
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http://hdl.handle.net/10362/180933 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 17192368 https://doi.org/10.3390/vaccines8010091 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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21 application/pdf |
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