Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs

Bibliographic Details
Main Author: Ladeira, Carina
Publication Date: 2023
Other Authors: Araújo, Rúben, Ramalhete, Luís, Teixeira, Hélder, Calado, Cecília R.C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/166843
Summary: Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL, IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL , IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Publisher Copyright: © 2023 The Authors
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spelling Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugsAntineoplasticsCytokinesis-blocked micronucleus assayFrozen bloodFTIR-spectroscopyGenotoxicityMolecular profileGeneticsHealth, Toxicology and MutagenesisFunding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL, IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL , IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Publisher Copyright: © 2023 The AuthorsGenotoxicity is an important information that should be included in human biomonitoring programmes. However, the usually applied cytogenetic assays are laborious and time-consuming, reason why it is critical to develop rapid and economic new methods. The aim of this study was to evaluate if the molecular profile of frozen whole blood, acquired by Fourier Transform Infrared (FTIR) spectroscopy, allows to assess genotoxicity in occupational exposure to antineoplastic drugs, as obtained by the cytokinesis-block micronucleus assay. For that purpose, 92 samples of peripheral blood were studied: 46 samples from hospital professionals occupationally exposed to antineoplastic drugs and 46 samples from workers in academia without exposure (controls). It was first evaluated the metabolome from frozen whole blood by methanol precipitation of macromolecules as haemoglobin, followed by centrifugation. The metabolome molecular profile resulted in 3 ratios of spectral bands, significantly different between the exposed and non-exposed group (p < 0.01) and a spectral principal component-linear discriminant analysis (PCA-LDA) model enabling to predict genotoxicity from exposure with 73 % accuracy. After optimization of the dilution degree and solution used, it was possible to obtain a higher number of significant ratios of spectral bands, i.e., 10 ratios significantly different (p < 0.001), highlighting the high sensitivity and specificity of the method. Indeed, the PCA-LDA model, based on the molecular profile of whole blood, enabled to predict genotoxicity from the exposure with an accuracy, sensitivity, and specificity of 92 %, 93 % and 91 %, respectively. All these parameters were achieved based on 1 μL of frozen whole blood, in a high-throughput mode, i.e., based on the simultaneous analysis of 92 samples, in a simple and economic mode. In summary, it can be conclude that this method presents a very promising potential for high-dimension screening of exposure to genotoxic substances.Comprehensive Health Research Centre (CHRC) - Pólo ENSPCentro de Investigação em Saúde Pública (CISP/PHRC)Escola Nacional de Saúde Pública (ENSP)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Comprehensive Health Research Centre (CHRC) - pólo NMSRUNLadeira, CarinaAraújo, RúbenRamalhete, LuísTeixeira, HélderCalado, Cecília R.C.2024-05-01T01:09:00Z2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/166843eng1383-5718PURE: 89588447https://doi.org/10.1016/j.mrgentox.2023.503681info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:20:58Zoai:run.unl.pt:10362/166843Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:51:45.947748Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
title Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
spellingShingle Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
Ladeira, Carina
Antineoplastics
Cytokinesis-blocked micronucleus assay
Frozen blood
FTIR-spectroscopy
Genotoxicity
Molecular profile
Genetics
Health, Toxicology and Mutagenesis
title_short Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
title_full Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
title_fullStr Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
title_full_unstemmed Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
title_sort Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
author Ladeira, Carina
author_facet Ladeira, Carina
Araújo, Rúben
Ramalhete, Luís
Teixeira, Hélder
Calado, Cecília R.C.
author_role author
author2 Araújo, Rúben
Ramalhete, Luís
Teixeira, Hélder
Calado, Cecília R.C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Centro de Investigação em Saúde Pública (CISP/PHRC)
Escola Nacional de Saúde Pública (ENSP)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Comprehensive Health Research Centre (CHRC) - pólo NMS
RUN
dc.contributor.author.fl_str_mv Ladeira, Carina
Araújo, Rúben
Ramalhete, Luís
Teixeira, Hélder
Calado, Cecília R.C.
dc.subject.por.fl_str_mv Antineoplastics
Cytokinesis-blocked micronucleus assay
Frozen blood
FTIR-spectroscopy
Genotoxicity
Molecular profile
Genetics
Health, Toxicology and Mutagenesis
topic Antineoplastics
Cytokinesis-blocked micronucleus assay
Frozen blood
FTIR-spectroscopy
Genotoxicity
Molecular profile
Genetics
Health, Toxicology and Mutagenesis
description Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL, IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL , IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Publisher Copyright: © 2023 The Authors
publishDate 2023
dc.date.none.fl_str_mv 2023-10
2023-10-01T00:00:00Z
2024-05-01T01:09:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/166843
url http://hdl.handle.net/10362/166843
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1383-5718
PURE: 89588447
https://doi.org/10.1016/j.mrgentox.2023.503681
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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